Lhcb4 | CP29 chlorophyll a/b binding protein of plant PSII
AS04 045 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, C. sativus, H. vulgare, N. tabacum, O. sativa, P. sativum, P. vulgaris, T. aestivum, Z. mays
|Recommended dilution||1 :7 000 (WB)|
|Expected | apparent MW||
31.9 | 29 kDa for Arabidopsis thaliana
|Confirmed reactivity||Arabidopsis thaliana, Cucumis sativus L. cv. Jihong no. 2, Drosera capensis, Hordeum vulgare, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Triticum aestivum, Zea mays
Catalpa bungei, Cucumis sativus, Populus, gymnosperms and microalgae Ostrecococcus tauri; the target sequence is only weakly conserved in Physcomitrella patens
|Not reactive in||
Chlamydomonas reinhardtii (please use AS06 117 for this organism)
|Selected references||Myouga et al. (2018). Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1) of the light harvesting-like family. Plant Physiol. 2018 Feb 1. pii: pp.01782.2017. doi: 10.1104/pp.17.01782. Kim et al. (2018). The rice zebra3 (z3) mutation disrupts citrate distribution and produces transverse dark-green/green variegation in mature leaves. Rice (N Y). 2018 Jan 5;11(1):1. doi: 10.1186/s12284-017-0196-8.
Tyuereva et al. (2017). The absence of chlorophyll b affects lateral mobility of photosynthetic complexes and lipids in grana membranes of Arabidopsis and barley chlorina mutants. Photosynth Res. 2017 Apr 5. doi: 10.1007/s11120-017-0376-9. (Hordeum vulgare, western blot)
Yang-Er Chen et al. (2017). Responses of photosystem II and antioxidative systems to high light and high temperature co-stress in wheat. J. of Exp. Botany, Volume 135, March 2017, Pages 45–55.
Betterle et al. (2016). The STN8 kinase-PBCP phosphatase system is responsible for high-light-induced reversible phosphorylation of the PSII inner antenna subunit CP29 in rice. Plant J. 2016 Nov 4. doi: 10.1111/tpj.13412. [Epub ahead of print]
Pavlovič et al. (2016). A carnivorous sundew plant prefers protein over chitin as a source of nitrogen from its traps. Plant Physiol Biochem. 2016 Mar 5;104:11-16. doi: 10.1016/j.plaphy.2016.03.008
Kim et al. (2015). Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation. Nat Commun. 2015 Apr 16;6:6843. doi: 10.1038/ncomms7843.
Sun et al. (2014). Direct energy transfer from the major antenna to the photosystem II core complexes in the absence of minor antennae in liposomes. Biochim Biophys Acta. 2014 Nov 22. pii: S0005-2728(14)00650-1. doi: 10.1016/j.bbabio.2014.11.005.
Grimmer et al. (2014). The RNA-binding protein RNP29 is an unusual Toc159 transport substrate. Front. Plant Sci. | doi: 10.3389/fpls.2014.00258
5 µg of total protein from embebed seeds of Nicotiana tabacum growing during 4 d in dark (0) and then transfer to continue light growing for 6 h (6) extracted with LB2x buffer and denatured 90 ºC for 2-5 min, were separated on 12.5 % SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with TBS-T with 5% dry-milk for 3h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 overnight at 4 ºC with agitation in TBS-T with 5% dry-milk. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4 times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1:30 000 in TBS-T with 5% dry-milk for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL Plus western blotting detection system by GE Healthcare. Exposure time was 60 seconds.
Courtesy of Dr. Concha Almoguera, Inst. de Recursos Naturales y Agrobiología –CSIC, Spain
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