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Lhcb4 | CP29 (Lhcb4) homolog (Ostreococcus tauri)

AS09 512 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Coccomyxa subellipsoidea, Ostreococcus tauri

Lhcb4 | CP29 (Lhcb4) homolog (Ostreococcus tauri) in the group Plant/Algal Antibodies / Photosynthesis  / LHC at Agrisera AB (Antibodies for research) (AS09 512)

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Background

Lhcb4 (CP29) is a member of the family of chlorophyll a/b-binding proteins, which is conserved in higher plants and green algae. Lhcb4 is associated with Photosystem II serving both as a light-harvesting antenna protein as well as playing a role in photoprotective dissipation of excitation energy. Alternative name: CP29-like light-harvesting chlorophyll a/b binding protein chlor (IC)

Immunogen

KLH-conjugated synthetic peptide derived from Lhcb4 (CP29) protein sequence from Ostreococcus tauri Q3B9U8

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS09 513 | anti-Lhcb5 | CP26 (Lhcb5) homolog, Ostreococcus tauri

LHC available antibodies against pigment-binding proteins

Algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW

27 | 27 kDa

Confirmed reactivity Coccomyxa subellipsoidea, Ostreococcus tauri
Predicted reactivity

Bathycoccus prasinos, Micromonas sp.,

Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references

Application example


Wstern blot using anti-Lhcb4 | CP29 (Lhcb4) homolog (Ostreococcus tauri) antibodies

1.0-4.5 µg of chlorophyll from Coccomyxa subellipsoidea cells were loaded to lanes. Samples were denatured with Laemmli buffer at 75°C for 5 min and were separated on 12% SDS-PAGE and blotted 30 min to PVDF using wet transfer. Blot was blocked with 5% milk in TBS for 2h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 overnight at 4°C with agitation in 1% milk in TBS-T. The antibody solution was decanted and the blot was washed 4 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in 1% milk in TBS-T for 1h at RT with agitation. The blot was washed 5 times for 5 min in TBS-T and 2 times for 5 min in TBS, and developed for 1 min with 1.25 mM luminol, 0.198 mM coumaric acid and 0.009% H2O2 in 0.1 M Tris- HCl, pH 8.5. Exposure time in ChemiDoc System was 115 seconds.

Courtesy MSc Paweł Rogowski, Warsaw University, Poland

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