Lhcb6 | CP24 chlorphyll a/b-binding protein of plant PSII
AS01 010 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Brassica napus, Hordeum vulgare
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27.5 | 24 kDa for Arabidopsis thaliana
Dictos, Gymnosperms, Physcomitrella patens, Pisum sativum, Selaginella martensii, Spinacia oleracea, Zea may
5 µg of total protein from Arabidopsis thaliana extracted with Agrisera Protein Extraction Buffer PEB (AS08 300) and denatured in PEB at 70°C for 5 min. were separated on 12% SDS-PAGE and blotted 1h to PVDF using semi-dry or tank transfer (blotted 15h to PVDF using tank-transfer - 30V). Blots were blocked with TBST with 4 % BSA for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation in TBS-T with 2% BSA. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1: 50 000 in for 1h at RT with agitation in TBS-T with 2% BSA. The blot was washed as above and developed with chemiluminescence detection reagent for 5 minutes. Exposure time was 25 seconds.
Courtesy of Dr. Robert Luciński, Department of Biology, UAM, Poznań, Poland
Lhcb6 is one of the 3 highly conserved minor chlorophyll a/b-binding proteins exclusively associated with Photosystem II in plants and algae. Together with Lhcb4 and Lhcb5, it regulates the energy flow from the outer antenna to the reaction center through the action of the xanthophyll cycle.
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