Lhcb6 | CP24 chlorphyll a/b-binding protein of plant PSII
AS01 010 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Brassica napus, Hordeum vulgare
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|Recommended dilution||1 : 1000-1 : 5000 (WB)|
|Expected | apparent MW||
27.5 | 24 kDa for Arabidopsis thaliana
|Confirmed reactivity||Arabidopsis thaliana, Brassica napus, Hordeum vulgare, Triticum aestivum
Dictos, Gymnosperms, Physcomitrella patens, Pisum sativum, Selaginella martensii, Spinacia oleracea, Zea may
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
Protein is processed into mature form (Jansson 1999).
This antibody is a re-make of former Lhcb6 antibody from Agrisera and is made to the same peptide.
|Selected references||Myouga et al. (2018). Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1) of the light harvesting-like family. Plant Physiol. 2018 Feb 1. pii: pp.01782.2017. doi: 10.1104/pp.17.01782. Wang et al. (2018). iTRAQ-based quantitative proteomics analysis of an immature high-oleic acid near-isogenic line of rapeseed. Molecular Breeding January 2018, 38:2.
Tyutereva et al. (2017). Stomata control is changed in a chlorophyll b-free barley mutant. Functional Plant Biology, doi.org/10.1071/FP17056
Chen et al. (2017). Comparison of Photosynthetic Characteristics and Antioxidant Systems in Different Wheat Strains. J Plant Growth Regul.
5 µg of total protein from Arabidopsis thaliana extracted with Agrisera Protein Extraction Buffer PEB (AS08 300) and denatured in PEB at 70°C for 5 min. were separated on 12% SDS-PAGE and blotted 1h to PVDF using semi-dry or tank transfer (blotted 15h to PVDF using tank-transfer - 30V). Blots were blocked with TBST with 4 % BSA for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation in TBS-T with 2% BSA. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1: 50 000 in for 1h at RT with agitation in TBS-T with 2% BSA. The blot was washed as above and developed for Clarity Western ECL Substrate Bio-Rad for 5 minutes. Exposure time was 25 seconds.
Courtesy of Dr. Robert Luciński, Department of Biology, UAM, Poznań, Poland
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