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LHCSR1

AS14 2819  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Bryopsis corticulans, Chlamydomonas reinhardtii, Nannochloropsis gaditana

LHCSR1 in the group Plant/Algal Antibodies / Photosynthesis  / LHC at Agrisera AB (Antibodies for research) (AS14 2819)

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product information
Background

LHCSR1 (Stress-related chlorophyll a/b binding protein 1) plays a role in an efficient enery dissipation process, called non-photochemical quenching (NPQ), in Chlamydomonas reinhardtii. 

Immunogen

KLH-conjugated synthetic peptide derived from LHCSR1 protein sequence from Chlamydomonas reinhardtii, UniProt: P93664

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized in PBS pH 7.4
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

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LHC  available antibodies against pigment-binding proteins

Collection of antibodies to Chlamydomonas proteins

Algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

27 kDa

Confirmed reactivity Bryopsis corticulans, Chlamydomonas reinhardtii, Nannochloropsis gaditana
Predicted reactivity please inquiry
Not reactive in Phaeodactylum tricornutum
Additional information
Selected references Kosuge et al.(2018). LHCSR1-dependent fluorescence quenching is mediated by excitation energy transfer from LHCII to photosystem I in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2018 Apr 3;115(14):3722-3727. doi: 10.1073/pnas.1720574115.
Giovagnetti et al. (2018). A siphonous morphology affects light-harvesting modulation in the intertidal green macroalga Bryopsis corticulans (Ulvophyceae). Planta. 2018 Feb 19. doi: 10.1007/s00425-018-2854-5.
Chukhutsina et al. (2017). Photoprotection strategies of the alga Nannochloropsis gaditana. Biochim Biophys Acta. 2017 Jul;1858(7):544-552. doi: 10.1016/j.bbabio.2017.05.003.
Allorent et al. (2016). UV-B photoreceptor-mediated protection of the photosynthetic machinery in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2016 Dec 20;113(51):14864-14869. doi: 10.1073/pnas.1607695114. Epub 2016 Dec 5.
Dinc et al. (2016). LHCSR1 induces a fast and reversible pH-dependent fluorescence quenching in LHCII in Chlamydomonas reinhardtii cells. Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):7673-8. doi: 10.1073/pnas.1605380113. Epub 2016 Jun 22.
Correa-Galvis et al. (2016). Photosystem II Subunit PsbS Is Involved in the Induction of LHCSR Protein-dependent Energy Dissipation in Chlamydomonas reinhardtii. J Biol Chem. 2016 Aug 12;291(33):17478-87. doi: 10.1074/jbc.M116.737312.

Application example
western blot using anti-LhcSR1 antibodies

10 ug of a total cell extract of Chlamydomonas reinhardtii: WT _P _HL_High CO2 (1), WT_P_HL (2),  Npq4_P _HL (3), were separated on Bolt® 4-12% Bis-Tris Plus Gels (precast) and blotted to PVDF using Bolt® transfer system for 1h . Blots were blocked with 5% BSA/milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and
3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 from Agrisera) diluted to 1:10 000 in for 1h at RT with
agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 10 seconds.
P: photoautotrophically grown cells
HL: High light illumination(light intensity:500 μE)

Courtesy Dr. Roberta Croce, Biophysics of Photosynthesis Dep. Physics and Astronomy Faculty of Sciences VU University Amsterdam, The Netherlands

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