Plastid acyl-ACP desaturase

AS13 2668  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii

Plastid acyl-ACP desaturase in the group Antibodies for Plant/Algal / Developmental Biology / Lipid metabolism at Agrisera AB (Antibodies for research) (AS13 2668)


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product information
Background Plastid acyl-ACP desaturase, coded by FAB2 gene is involved in fatty acid metabolism in Chlamydomonas reinhardtii. It displays acyl-[acyl-carrier-protein] desaturase activity. 

putative mature form of Chlamydomonas reinhardtii plastid acyl-ACP desaturase expressed in E.coli,  UniProt: A8IQB8

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
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Additional information
application information
Recommended dilution 1 : 1000-1 : 1500 (WB)
Expected | apparent MW

40 | 35 kDa

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity Volvox carteri
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information Antibody dilution is going to be higher with ECL .
Selected references

to be added when available, antibody released in May 2014

application example 

western blot using FAB2 antibody
20 µg of soluble protein from Chlamydomonas reinhardii, were separated on 10 % SDS-PAGE and blotted for 1h to nitrocellulose. Blots were blocked with 1 % milk in PBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the plastid acyl-ACP desaturase antibody in 1 % milk in PBS-T at a dilution 1: 1500 for 2h at RT with agitation (left panel) or pre-immune serum (right panel). The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG, ALP conjugated) diluted to 1:3 000 in  for 1h at RT with agitation. The blot was washed as above and reaction was visualized using BCIP/NBT. 

Courtesy Dr. Dudley Page, UCLA, USA

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