FOX1 | plasma membrane ferroxidase FLP1

AS06 120 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii


product information
Background		A copper-containing protein in Chlamydomonas reinhardtii is a plasma membrane ferroxidase, encoded by the FOX1 gene, involved in Fe assimilation. FOX1 is most highly related to the mammalian homologues hephaestin and ceruloplasmin; its occurrence and pattern of expression in Chlamydomonas indicate a role for copper in iron assimilation in a photosynthetic species.
Immunogen		residues 394-646 from Chlamydomonas reinhardtii FOX1 A8IZT9 fused to TrxA
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Lyophilized
Quantity		200 µl
Reconstitution		For reconstitution add 200 µl of sterile water.
Storage		Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		immunofluorescence (IF), Western blot (WB)
Related products		AS07 260 \| anti-H+ATPase, plasma membrane rabbit marker antibody Collection of antibodies to Chlamydomonas proteins Algal protein extraction buffer Secondary antibodies
Additional information		FOX1 is 10-20-fold induced in iron-deficiency and its occurrence therefore serves as a marker of iron nutrition state in Chlamydomonas. For detection image please refer to the article below.

application information
Recommended dilution		1 : 1000-1 : 5000 (WB)
Expected \| apparent MW		122 \| 120 kDa
Confirmed reactivity		Chlamydomonas reinhardtii
Predicted reactivity		Chlamydomonas reinhardtii
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information		This antibody has been used on Chlamydomonas cells expressing endogenous Fox1 and on HEK293 cells transiently expressing Fox1. Cells were resuspended in PBS plated on poly-lysine coated coverslips, fixed in 3.7% formaldehyde. Blocked in 3% BSA in PBS. 1:500 dilution of anti-FOX1 in 3% BSA. Secondary antibody was anti-rabbit Alexa fluor 488 1:300.
Selected references		Kropat et al. (2015). Copper economy in Chlamydomonas: Prioritized allocation and reallocation of copper to respiration vs. photosynthesis. Proc Natl Acad Sci U S A. 2015 Feb 2. pii: 201422492. LaFontaine et al. (2002) Copper-Dependent Iron Assimilation Pathway in the Model Photosynthetic Eukaryote Chlamydomonas reinhardtii. Eukaryotic Cell 1(5):736-757

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