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Epitope tag Antibodies


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PGRL1 | PGR5-like protein 1A (chloroplastic)

AS10 725 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Spinacia oleracea

PGRL1 | PGR5-like protein 1A (chloroplastic) in the group Antibodies for Plant/Algal / Photosynthesis / Light acclimation at Agrisera AB (Antibodies for research) (AS10 725)

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product information
Background

PGRL1 is a transmembrane protein present in thylakoids of higher plants and algae. Arabidopsis plants lacking PGRL1 show perturbation of cyclic electron transport (CEF) around photosystem I (PSI), similar to PGR5-deficient plants. PGRL1 has been shown to interact physically with PGR5 and associate with PSI (DalCorso et al., 2008). In Chlamydomonas reinhardtii, PGRL1 is part of a protein supercomplex, composed of PSI with its own light-harvesting complex (LHCI), the photosystem II light-harvesting complex (LHCII), the cytochrome b6/f complex (Cyt b6f), ferredoxin (Fd)-NADPH oxidoreductase (FNR), responsible of the energy balance of the two photosystems and of the switch between thylakoid linear and cyclic electron transport (Iwai et al., 2010). Synonymes:
Immunogen

the N-terminal loop (aa 61 to 200; N-PGRL1) of PGRL1A (Q8H112, At4g22890). The N-PGRL1 protein was purified by virtue of its His-Tag using Ni-NTA Agarose (QIAGEN).
Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

collection of antibodies to photosynthetic proteins

Plant and algal protein extraction buffer

Secondary antibodies
Additional information
application information
Recommended dilution 1 : 3000 (WB)
Expected | apparent MW

29 | 29 kDa (Arabidopsis thaliana, Spinacia oleracea)
Confirmed reactivity Arabidopsis thaliana, Spinacia oleracea
Predicted reactivity Cucumis sativus, Glycine max, Ricinus communis, Vitis vinifera, Populus trichocarpa, Oryza sativa, Zea mays
Not reactive in

Chlamydomonas reinhardtii, Nicotiana tabacum, Physcomitrella patens
Additional information

In Arabidopsis thaliana samples cross-reactivity in the region of 55-60 kDa can be detected.

This product can be sold containing ProClin if requested
Selected references Nikkanen et al. (2018). Regulation of chloroplast NADH dehydrogenase-like complex by NADPH-dependent thioredoxin system. CSH, BioRixiv. doi.org/10.1101/261560.
Lintala et al. (2013). Arabidopsis tic62 trol mutant lacking thylakoid bound ferredoxin-NADP+ oxidoreductase shows distinct metabolic phenotype. Mol Plant Sep 16.
Endow and Inoue (2013).Stablecomplexformation ofthylakoidalprocessingpeptidase andPGRL1. FEBS Lett. May 31st.

application example

5 µg of chlorophyl a/lane from Arabidopsis thaliana wild-type thylakoids (1), Arabidopsis thaliana pgr5 mutant thylakoids (2), Arabidopsis thaliana double mutant thylakoids (3), were separated on 12% SDS-PAGE and blotted 1h to nitrocellulose membrane.Blots were blocked immediately following transfer with 5% dry milk in 1x TBS together with 0.1% Tween-20 (1x TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1:3000 for 2h at room temperature with agitation. The antibody solution was decanted and the blot washed three times, 10 min each, with 1x TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit horseradish peroxidase conjugated, from Pierce) diluted to 1:50.000 for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL detection reagent according to the manufacturer’s instructions. Exposure time was 5 min.

Courtesy Paolo Pesaresi, Alexander Hertle, Roberto Barbato, Dario Leister, Ludwig-Maximilians-University Munich

 

 

western blot using anti-PGRL1 antibodies

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