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AGO set
Tag Antibody Set 

ClpC | Chloroplastic form of HSP100

AS01 001  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A.thaliana, C.reinhardtii, cyanobacteria, Plectonema sp., Streptomyces sp.

ClpC | Chloroplastic form of HSP100 in the group Antibodies for Plant/Algal  / Photosynthesis  / Proteases at Agrisera AB (Antibodies for research) (AS01 001)

PRODUCT INFORMATION IN PDF


Datasheet Product citations Protocols Customer reviews

Product Information

Immunogen

Recombinant ClpC (C-terminal domain overexpressed as fusion with maltose-binding protein) Q55023

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 1000 (IHC), 1 : 5000 on 10 µg of total protein, (WB)
Expected | apparent MW

92 | 87 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Synechococcus sp., Chlamydomonas reinhardtii, Streptomyces sp.
Predicted reactivity

Algae (red), Catalpa bungei, Nicotiana tabacum, Ostreococcus sp., Oryza sativa, Populus trichocarpa,  Physcomitrella patens, Pisum sativum, Solanum tuberosum,  Zea mays

Not reactive in Different strains of Mycobacterium smegmatis

Application examples

Additional information

Additional information

Anti-ClpC antibodies will also recognized Arabidopsis thaliana isoform ClpC1 (At5g50920) and ClpC2 (At3g48870).

Related products

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AS9 459 | anti-ClpB-P | ClpB3, rabbit antibodies

AS13 2655 | anti-ClpP6 | chloroplast ClpP6 proteolytic subunit, rabbit antibodies
AS15 3077 | anti-pClpP | Chloroplastic ATP-dependent Clp protease proteolytic subunit 1, rabbit antibodies

AS07 253 | anti-HSP101 | ClpB heat shock protein, N-terminal, rabbit antibodies
AS08 287 | anti-HSP101 | ClpB heat shock protein, C-terminal, rabbit antibodies

AS08 355 | anti-slr0156 | ATP-dependent chaperone clpB, rabbit antibodies
AS08 344 | anti-slr1641 | ATP-dependent chaperone clpB, rabbit antibodies

Background

Background

ClpC is a chloroplastic protein of the Hsp100 family. It is believed to function as a housekeeping enzyme, both in its capacity as an independent molecular chaperone and as the regulatory component of the Clp protease.

Product citations

Selected references Hu et al. (2015). Site-specific Nitrosoproteomic Identification of Endogenously S-Nitrosylated Proteins in Arabidopsis. Plant Physiol. 2015 Feb 19. pii: pp.00026.2015.
Rosano et al. (2011). Insights into the Clp/HSP100 chaperone system from chloroplasts of Arabidopsis thaliana. J Biol Chem. Aug 26;286(34):29671-80. (Western blot, Arabidopsis thaliana)
Karradt et al. (2008) NblA, a Key Protein of Phycobilisome Degradation, Interacts with ClpC, a HSP100 Chaperone Partner of a Cyanobacterial Clp Protease. J Biol Chem 283: 32394-32403.
Porankiewicz & Clarke (1997) Induction of the heat shock protein ClpB affects cold acclimation in the cyanobacterium Synechococcs sp. strain PCC7942. J Bacteriol 179:5111-5117.

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