pClpP | Chloroplastic ATP-dependent Clp protease proteolytic subunit 1

361 €

AS15 3077  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, H. vulgare, S. lycopersicum, S. oleracea,


17 st
Item No:
AS15 3077

Info: Product suggestions Add review
product information
Background pClpP (Chloroplastic ATP-dependent Clp protease proteolytic subunit 1) has a chymotrypsin-like activity and plays a major role in the degradation of misfolded proteins.
Immunogen Full length, recombinant pClpP protein from Arabidopsis thaliana, UniPro: P56772, TAIR: ATCG00670
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products AS08 344 | anti-ClpB1 ATP-dependent chaperone, rabbits antibodies
AS08 355 |anti-ClpB2 ATP-dependent chaperone, rabbit antibodies

AS9 459 | anti-ClpB-P | ClpB3, rabbit antibodies

AS01 001
| anti-ClpC | chloroplastic form of HSP100, rabbit antibodies
AS13 2655 | anti-ClpP6 | chloroplast ClpP6 proteolytic subunit, rabbit antibodies

AS07 253 | anti-HSP101 | ClpB heat shock protein, N-terminal, rabbit antibodies
AS08 287 | anti-HSP101 | ClpB heat shock protein, C-terminal, rabbit antibodies

AS08 355 | anti-slr0156 | ATP-dependent chaperone clpB, rabbit antibodies
AS08 344 | anti-slr1641 | ATP-dependent chaperone clpB, rabbit antibodies
Additional information
application information
Recommended dilution 1 : 5 000 (WB)
Expected | apparent MW

22 kDa

Confirmed reactivity Arabidopsis thaliana, Nicotiana tabcum, Spinacia oleracea, Solanum lycopersicum
Predicted reactivity Aethionema grandiflorum, Arabis hirsuta, Barbarea verna, Brassica napus, Capsella bursa-pastoris, Cardamine impatiens, Carica papaya, Coffea arabica, Crucihimalaya wallichii, Daucus carota, Draba nemorosa, Ehretia acuminata, Ellisia nyctelea, Forsythia europaea, Hesperelaea palmeri, Lepidium virginicum, Lobularia maritima, Nasturtium officinale, Nicotiana tabacum, Olea europaea subsp. maroccana, Olimarabidopsis pumila , Pachycladon cheesemanii, Raphanus sativus, Scutellaria baicalensis
Not reactive in

Hordeum vulgare

Additional information
Selected references To be added when available, antibody released in December 2015

application example

western blot using anti-ClpP antibodies
20 µg of total protein from leaf extract from Spinacia oleracea (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) leafs were separated on 12 % SDS-PAGE  and blotted 1h to PVDF using tank transfer. Blots were blocked with 5 % milk in TBS  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary anti-ClpP antibody at a dilution of 1: 5 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 2 min with ECL according to the manufacturer's instructions. Exposure time was 5 seconds.

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at