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AGO set
Tag Antibody Set 

Hcf101 | High chlorophyll fluorescence phenotype protein

AS06 163 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana | cellular [compartment marker] of plastid stroma

Hcf101 | High chlorophyll fluorescence phenotype protein in the group Antibodies for Plant/Algal  / Photosynthesis  / PSI (Photosystem I) at Agrisera AB (Antibodies for research) (AS06 163)

PRODUCT INFORMATION IN PDF


Datasheet Product citations Protocols Add review

Product Information

Immunogen

recombinant Arabidopsis thaliana Hcf101, amino acid residues 206-532 Q6STH5, At3g24430

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunolocalization (IL), Western blot (WB)
Recommended dilution 1 : 3000 (IL), 1 : 3000 (WB)
Expected | apparent MW

57 | 50.5 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity

Arabidopsis thaliana

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information Cellular [compartment marker] of plastid stroma

Immunolocalization has been done on isolated plastids, followed by separation into stroma and thylakoids

Related products

Related products

AS03 037 | Anti-RbcL stroma, rabbit marker antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Background

Hcf101 is a novel protein required for photosystem I biogenesis. Hcf101-1 is a high-chlorophyll-fluorescence hcf) Arabidopsis thaliana mutant that lacks photosystem I. In green tissue the level of this protein is stimulated by light and the protein has not been detected in roots. It has been localized in chloroplast stroma.

Product citations

Selected references Hu et al. (2017). The SUFBC2 D Complex is Required for the Biogenesis of All Major Classes of Plastid Fe-S Proteins. Plant J. 2017 Jan 19. doi: 10.1111/tpj.13483.
Bigeard et al. (2014). Proteomic and phosphoproteomic analyses of chromatin-associated proteins from Arabidopsis thaliana. Proteomics. 2014 May 31. doi: 10.1002/pmic.201400072.

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