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Agrisera Super Deal

Primary/Secondary/ECL



Add only 20 € to your primary antibody purchase
and you will also receive:

- Secondary antibody
Goat anti-rabbit, HRP conjugated
(to be used >1: 25 000)


- Two chemiluminescent detection reagents
Agrisera ECL Bright/SuperBright,  for 10 midi blots (picogram and femtogram detection range)

AS18 PrimarySecondaryECL

PsaG | PSI-G subunit of photosystem I

AS04 048  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, N. tabacum, S. oleracea

PsaG | PSI-G subunit of photosystem I in the group Plant/Algal Antibodies / Photosynthesis  / PSI (Photosystem I) at Agrisera AB (Antibodies for research) (AS04 048)

PRODUCT INFORMATION IN PDF

Qty: 
332 €

Info: Add review
product information
Background

PsaG is subunit located in the Photosystem I complex. It plats a role in stablizing the binding of the peripheral antenna. PsaG, together with PsaH and PsaN, are unique to higher plants and algae. Immunogen: Fusion protein between DHFR and the mature part of PsaG.

Immunogen

Fusion protein between DHFR and the mature part of PSI-G (Arabidopsis thaliana, accession At1g55670 in the pQE42 vector

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB), Immunogold (IG)
Related products

antibody collection to PSI proteins

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 120-1 : 500 (IG), 1 : 2000-1 : 5000 (WB)
Expected | apparent MW

17 | 11 kDa

Confirmed reactivity Arabidopsis thaliana, Nicotiana tabcum, Spinacia oleracea
Predicted reactivity

Pisum sativum

Not reactive in

Hordeum vulgare, Chlamydmonas reinhardtii, Synechococcus sp. 7842

Additional information Immunogold localization has been done in leaf material of Arabidopsis thaliana.
Selected references Bock (2012). The plastid genome-encodedYcf4 protein functions as a non-essential assembly factor for photosystem I in higher plants. Plant Physiol. ahead of print.
Jensen et al. (2002) Photosystem I activity is increased in the absence of the PSI-G subunit. J. Biol. Chem. 277: 2798-2803.

application example

2 µg of total protein from  (1) Arabidopsis thaliana leaf extracted with PEB (AS08 300),(2) Horderum vulgare leaf extracted with PEB (AS08 300), (3) Chlamydomonas reinhardtii total cell extracted with PEB (AS08 300), (4) Synechococcus sp. 7942 total cell extracted with PEB (AS08 300) were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:20 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).

 

Western blot detection using anti-PsaG antibodies 

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com