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Agrisera Super Deal

Primary/Secondary/ECL



Add only 20 € to your primary antibody purchase
and you will also receive:

- Secondary antibody
Goat anti-rabbit, HRP conjugated
(to be used >1: 25 000)


- Two chemiluminescent detection reagents
Agrisera ECL Bright/SuperBright,  for 10 midi blots (picogram and femtogram detection range)

AS18 PrimarySecondaryECL

PsaL | PSI-L subunit of photosystem I

AS06 108  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, H. vulgare, N. tabaccum, S. oleracea

PsaL | PSI-L subunit of photosystem I in the group Plant/Algal Antibodies / Photosynthesis  / PSI (Photosystem I) at Agrisera AB (Antibodies for research) (AS06 108)

PRODUCT INFORMATION IN PDF

Qty: 
355 €

Info: Add review
product information
Background

PsaL (PSI-L) is a conserved subunit of type I photosynthetic reaction centers (Photosystem I, PSI). PSI is an integral membrane multi-protein complex that catalyzes the electron transfer from plastocyanin (or cytochrome c6) to ferredoxin (or flavodoxin). Psa-L is binding pigments and has been shown to be involved in trimerization of PSI in cyanobacteria (but not in plants) and bind pigments in plants and cyanobacteria.In plants and algae Psa-L is nuclear encoded and imported post-translationally into the chloroplast where it inserts into the thylakoid membrane.

Immunogen

KLH-conjugated synthetic peptide derived from PsaL protein sequence from Arabidopsis thaliana (At4g12800). This sequence is well conserved in most mono- and dicots but not in Physcomitrella patens.

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

PSI available antibodies to Photosystem I proteins

Photosynthesis available antibodies to photosynthetic proteins

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

18 | 17-18 (Arabidopsis thaliana)

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabaccum, Spinacia oleracea
Predicted reactivity Dicots, Monocots
Not reactive in Cyanobacteria
Additional information
Selected references Sook Seok et al. (2013). AtFKBP16-1, a chloroplast lumenal immunophilin, mediates response to photosynthetic stress by regulating PsaL stability. Physiologia Plantarum, DOI: 10.1111/ppl.12116.
Bock (2012). The plastid genome-encodedYcf4 protein functions as a non-essential assembly factor for photosystem I in higher plants. Plant Physiol. ahead of print.

application example

2 µg of total leaf protein of Arabidopsis thaliana (1) and Hordeum vulgare (2) and total cellular protein of Chlamydomonas reinhardtii (3) and Synechococcus PCC 7942 (4) isolated with PEB (AS08 300) were separated on 4-12% Nupage Bis-Tris gels in in MES running buffer (Invitrogen) at 200V for 35 minutes. Proteins were transferred for 80 minutes at 30V to a PVDF membrane pre-wetted in methanol and equilibrated in 1X transfer buffer. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) and probedwith anti-PsaL (AS06 148 1:1000) and secondary HRP-conjugated goat anti-rabbit antibody (1:50 000) for 1 hr in TBS-T containing 2% ECL Advance blocking reagent (GE Healthcare). Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signals was detected  using ECL Advance detection reagent (GE Healthcare) according to the manufacturers instructions and a CCD imager (FluorSMax, Bio-Rad)

 

 

 


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