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AGO set
Tag Antibody Set 

Ycf3 | Photosystem I assembly protein ycf3

AS07 273  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii, cyanobacteria

Ycf3 | Photosystem I assembly protein ycf3 in the group Antibodies for Plant/Algal  / Photosynthesis  / PSI (Photosystem I) at Agrisera AB (Antibodies for research) (AS07 273)

PRODUCT INFORMATION IN PDF

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Datasheet Product citations Add review

Product Information

Immunogen

full length recombinant ycf3 protein of Chlamydomonas reinhardtii  O20031, as described in Boudreau et al. 1997

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

19 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii, cyanobacteria
Predicted reactivity Algae, Chlorella vulgaris, Marchantia polymorpha, Physcomitrella patens, Chlorokybus atmophyticus, Ostreococcus tauri
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Western blot detection image can be found in Boudreau et al. 1997.

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Background

Background

Ycf3 together with Ycf4 have been found as extrinistic thylakoid membrane proteins which are required for the accumulation of PSI complex in Chlamydomonas reinhardii.

Product citations

Selected references Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113. Epub 2016 Feb 22.
Naver et al. (2001). Functional studies of Ycf3. The Plant Cell 13:2731- 2746.
Boudreau et al. (1997) The chloroplast ycf3 and ycf4 open reading frames of Chlamydomonas reinhardtii are required for the accumulation of the photosystem I complex. The EMBO J.16:6095-6104.