PSII (Photosystem II)
PSI/PSII ratio can be estimated once a quantitative immunoblot for PsaC (AS10 939) and for either PsbA (AS05 084) or PsbD (AS06 146), is performed in 3-4 replicates. Ratio calculation is done on a sample by sample basis. These ratios are based on the subunits present, whether they are in the assembled photosystem or not. Under some circumstances (eg. high light), there are biological reasons that the subunit numbers do not necessarily match the functional photosystems.
Literature
Brown et al. (2008). Flux capacities and acclimation costs in Trichodesmium from the Gulf of Mexico. Marine Biology 154 (3): 413-422.
Levitan et al. (2010). Regulation of nitrogen metabolism in the marine diazotroph Trichodesmium IMS101 under varying temperatures and atmospheric CO2 concentrations. Environmental Microbiology 12(7): 1899-1912.
Thamatrakoln et al. (2013). A ‘Death-Specific Protein’ in a marine diatom regulates photosynthetic responses to iron and light availability. Proceedings of the National Academy of Science 110(50): 20123-20128 Li G,
Li et al. (2014). The Nitrogen Costs of Photosynthesis in a Diatom under Current and Future pCO2. New Phytologist, 205:533-543.
Protocols and Technical Information • Support • Western Blot Video Tutorials
AS06 157 | Clonality: Polyclonal | Host: Rabbit | Reactivity: plants including A. balsamea, A.thaliana, H.vulgare, P.strobus, S.oleracea, Synechococcus sp. PCC 7942, Z.mays
AS10 692 | Clonality: Polyclonal | Host: Rabbit | Reactivity: cyanobacteria
AS06 158 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, H.vulgare
AS14 2786 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
AS05 092 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, N. tabacum, P. sativum, Z. mays
AS21 4689 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii, Synechococcus elongatus, Synechocystis sp.
AS14 2824 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana
AS14 2825 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana
AS08 305 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, S. bicolor, Z. mays
AS06 167 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, N. tabacum, P.abies, P. sativum, P. patens
AS06 142-23 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, Ch. reinhardtii, P. banksiana. , S.oleracea, T. aestivum
AS10 682 | Clonality: Polyclonal | Host: Rabbit | Reactivity: cyanobacteria
AS06 142-16 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, P. abies, T. aestivum, Z. mays
AS05 059 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, N. tabacum, P. sativum, S. oleracea, T. aestivum
AS03 032 | Clonality: Polyclonal | Host: Hen | Reactivity: A. thaliana, A. sativa, B. napus, H. vulgare, O. sativa, P. sylvestris, P. tremula, S. oleracea
AS09 533 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. corticulans, B. distachyon, C. cantabricus (Wilk.) Rchb. F, F.a bidentis, H. pilosella L., H. vulgare, L. hispanica, M. esculenta, N. tabacum, O. sativa, P. abies, P. glauca, P. strobus, R.communis, S.oleracea, S. muralis (Hedw.) Raab, T. aestivum, Z.mays
AS06 171 | Clonality: | Polyclonal | Host: Rabbit | Arabidopsis thaliana
AS05 060 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Nicotiana tabacum, Spinacia oleracea
AS05 066 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana
AS06 114 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
AS08 369 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, Z.mays