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PsbF | beta subunit of Cytochrome b559 of PSII

AS06 113 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, P.sativum, T. aestivum

PsbF | beta subunit of Cytochrome b559 of PSII in the group Antibodies for Plant/Algal / Photosynthesis  / PSII (Photosystem II) at Agrisera AB (Antibodies for research) (AS06 113)

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product information
Background

Cytochrome b559 (Cyt b559) is encoded by the chloroplast genes psbE and psbF and is comprised of two low molecular mass polypeptides, α and ß subunits, with molecular masses of 9 and 4 kDa, respectively. The Cyt b559 is closely associated with PSII in all oxygenic photosynthetic organisms. The α and ß subunits of the Cyt b559 are components of the minimal PSII reaction center complex that is still capable of primary charge separation In summary, both PsbE and PsbF are essential components for PSII assembly, and they are probably involved in electron transport mechanisms that help to protect PSII from photodamage.

Immunogen

KLH-conjugated synthetic peptide derived from plant PsbF sequences including Arabidopsis thaliana P62095, AtCg00570

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

collection of antibodies to PSII proteins

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

4 kDa

Confirmed reactivity Arabidopsis thaliana, Pisum sativum, Triticum aestivum
Predicted reactivity Cannabis sativa, Nicotiana tabacum, Pinus sylvestris, Populus tremula
Not reactive in

Cyanobacteria

Additional information

This antibody works better on thylakoid and chloroplast fractions than on a total cell extract.

Selected references Hackett et al. (2017). An Organelle RNA Recognition Motif Protein Is Required for Photosystem II Subunit psbF Transcript Editing. Plant Physiol. 2017
Yang-Er Chen et al. (2017). Responses of photosystem II and antioxidative systems to high light and high temperature co-stress in wheat. J. of Exp. Botany, Volume 135, March 2017, Pages 45–55.
Nishimura et al. (2016). The N-terminal sequence of the extrinsic PsbP protein modulates the redox potential of Cyt b559 in photosystem II. Sci Rep. 2016 Feb 18;6:21490. doi: 10.1038/srep21490.
Lucinski et al. (2011). Involvement of Deg5 protease in wounding-related disposal of PsbF apoprotein. Plant Physiol Biochem. 49(3):311-20.
Garcia-Cerdan et al. (2008). Antisense inhibition of the PsbX protein affects PSII integrity in the higher plant Arabidopsis thaliana. Plant Cell Physiol 50: 191-202

application example

5µg protein of Arabidopsis thaliana (1) leaf, extracted with PEB (AS08 300), (2) chloroplasts (normal light), (3) thylakoids (extracted from 2), (4) chloroplasts (high light), (5) thylakoids (extracted from 4), (6) Photosystem I, and (7) BBY particles (enriched in Photosystem II) from pea were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1.5h (30V) to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with AS06 113 (1:1000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare) using a Fuji LAS-3000 CCD (150s, high sensitivity). The PsbF protein is detected with an apparent mass of < 10 kDa, the antibody cross-reacts with a target at ~43 kDa.

 

 

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