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AGO set
Tag Antibody Set 

PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII (anti-protein)

AS06 142-23  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, Ch. reinhardtii, P. banksiana. , S.oleracea, T. aestivum

PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII (anti-protein) in the group Antibodies for Plant/Algal  / Photosynthesis  / PSII (Photosystem II) at Agrisera AB (Antibodies for research) (AS06 142-23)

PRODUCT INFORMATION IN PDF

Qty: 
355 €

Datasheet Product citations Protocols Add review

Product Information

Immunogen

native, purified 23 kDa protein from Spinacia oleracea,UniProt: P12302

Host Rabbit
Clonality Polyclonal
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 260 µg
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 2000-1 : 5000 (WB)
Expected | apparent MW

28 | 23 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Chlamydomonas reinhardti, Hordeum vulgare, Pinus banksiana, Spinacia oleracea, Triticum aestivum
Predicted reactivity Musa acuminata, Oryza sativa, Pinus monticola, Pisum sativum, Populus balsamifera, Solanum lycopersicum
Not reactive in

Synechococcus sp. PCC 7942

Application examples

Application examples

Application example

western blot using anti-PsbP antibodies

2 µg of total protein from Arabidopsis thaliana leaf (1)  Hordeum vulgare leaf (2),  Chlamydomonas reinhardtii total cell (3)Synechococcus sp. 7942 total cell (4),   Anabaena sp. total cell (5), all extracted with PEB (AS08 300) and separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2%  blocking reagent in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in 2% blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with chemiluminescent detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).

Additional information

Load per well on cell extract of Pinus banksiana (Jack Pine) was 7 µg.

This antibody can be used as a loading control for Chlamydomonas reinhardtii while it not so suitable for higher plants as accumulation of these proteins might drop to 12.5-25 % of the WT level in mutants defective for PSII core (Schult et al. 2007).

Related products

Related products

AS05 092 | Anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibodies

AS06 142-33 | Anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibodies

AS06 167 | Anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibodies

AS08 305 | Anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibodies

AS06 142-16 | Anti-PsbQ | 16 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Background

PSII reaction centre components are  generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site          of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.

Product citations

Selected references Nama et al. (2018). Non-photochemical quenching-dependent acclimation and thylakoid organization of Chlamydomonas reinhardtii to high light stress. Photosynth Res. 2018 Jul 7. doi: 10.1007/s11120-018-0551-7.
Yang-Er Chen et al. (2017). Responses of photosystem II and antioxidative systems to high light and high temperature co-stress in wheat. J. of Exp. Botany, Volume 135, March 2017, Pages 45–55.
Wang et al. (2008). Beta-lactone probes identify a papain-like peptide ligase in Arabidopsis thaliana. Nat Chem Biol. 4: 557-563.

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