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PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII (anti-monocot protein)

AS08 305  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, S. bicolor, Z. mays

PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII (anti-monocot protein) in the group Antibodies for Plant/Algal  / Photosynthesis  / PSII (Photosystem II) at Agrisera AB (Antibodies for research) (AS08 305)

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Product Information

Immunogen

Recombinant sorghum OE23 protein

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 5 000 (WB)
Expected | apparent MW

23 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Sorghum bicolor, Zea mays
Predicted reactivity Hordeum vulgare, Oryza sativa
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

AS08 305 PsbP western.jpg

 
 
5 µg of total protein from (1) Arabidopsis thaliana total leaf extract; (2) Zea mays total leaf  extract were separated in a 12% or 5-15% gradient gel following by transfer to nitrocellulose membrane. Membrane was blocked with TBST + 4% non-fat dried milk, 20 min following by three washes in TBST. Incubation time with primary and secondary antibodies was 1 hr primary, 30 min for secondary antibodies. Dectetion was performed using chemiluminescence. 

Additional information

Related products

Related products

AS05 092 | Anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibodies

AS06 142-33 | Anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibodies

AS06 167 | Anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibodies

AS06 142-16 | Anti-PsbQ | 16 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibodies

Background

Background

PSII reaction centre components are  generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.

Product citations

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