PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII (anti-monocot protein)
AS08 305 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, S. bicolor, Z. mays
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Recombinant sorghum OE23 protein
5 µg of total protein from (1) Arabidopsis thaliana total leaf extract; (2) Zea mays total leaf extract were separated in a 12% or 5-15% gradient gel following by transfer to nitrocellulose membrane. Membrane was blocked with TBST + 4% non-fat dried milk, 20 min following by three washes in TBST. Incubation time with primary and secondary antibodies was 1 hr primary, 30 min for secondary antibodies. Manufacturer of secondary antibodies: Bio-Rad
PSII reaction centre components are generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.
to be added when available