Name:
Phone:
E-mail:
Message

Agrisera Super Deal

Primary/Secondary/ECL



Add only 20 € to your primary antibody purchase
and you will also receive:

- Secondary antibody
Goat anti-rabbit, HRP conjugated
(to be used >1: 25 000)


- Two chemiluminescent detection reagents
Agrisera ECL Bright/SuperBright,  for 10 midi blots (picogram and femtogram detection range)

AS18 PrimarySecondaryECL

PsbW | Small subunit W of PSII

AS05 060  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Nicotiana tabacum, Spinacia oleracea

PsbW | Small subunit W of PSII in the group Plant/Algal Antibodies / Photosynthesis  / PSII (Photosystem II) at Agrisera AB (Antibodies for research) (AS05 060)

PRODUCT INFORMATION IN PDF

Qty: 
355 €

Info: Add review
product information
Background

PsbW is a nuclear-encoded protein located in the thylakoid membrane of the chloroplast. It is a core component of Photosystem II. Altrnative name: PSII 6.1 kDa protein

Immunogen

KLH-conjugated synthetic peptide derived from PsbW protein sequence of Arabidopsis thaliana At2g30570

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

collection of antibodies to PSII proteins

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 15 000 (WB)
Expected | apparent MW

13.6 | 6.1 kDa

Confirmed reactivity Arabidopsis thaliana, Horderum vulgare, Nicotiana tabacum, Spinacia oleracea
Predicted reactivity Oryza sativa, Physcomitrella patens, Zea mays, Vitis vinifera
Not reactive in

Chlamydomonas reinhardtii, Synechococcus sp. PCC 7942

Additional information
Selected references Hackett et al. (2017). An Organelle RNA Recognition Motif Protein Is Required for Photosystem II Subunit psbF Transcript Editing. Plant Physiol. 2017 Apr;173(4):2278-2293. doi: 10.1104/pp.16.01623.

application example

2 µg of total protein from (1) Arabidopsis thaliana leaf, (2) Horderum vulgare leaf ), (3) Chlamydomonas reinhardtii total cell , (4) Synechococcus sp. 7942 total cell were all extracted with PEB (AS08 300) and separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 50 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).  Exposure time was 1 second.

 

  western blot detection using PsbW antibody

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com