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AGO set
Tag Antibody Set 

PsbZ | ycf9 protein of PSII

AS06 115  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Pisum sativum

PsbZ | ycf9 protein of PSII in the group Antibodies for Plant/Algal  / Photosynthesis  / PSII (Photosystem II) at Agrisera AB (Antibodies for research) (AS06 115)

PRODUCT INFORMATION IN PDF

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355 €

Datasheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from PsbZ protein sequence of Arabidopsis thaliana (AtCg00300). This sequence is highly conserved in dicots, monocots, and Physcomitrella patens but only weakly in Chlamydomonas reinhardtii.

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

6.5 | 6.5 kDa for Arabidopsis thaliana

Reactivity

Confirmed reactivity Arabidopsis thaliana, Pisum sativum
Predicted reactivity

Dicots, Physcomitrella patens

Not reactive in

Cyanobacteria

Application examples

Application examples

Application example

Western blot detection using PsbZ antibody

5 µg of total protein from (1) Arabidopsis thaliana leaf extracted with PEB (AS08 300), (2) chloroplast, (3) thylakoids, (4) chloroplasts from high light treated plants, (5) thylakoids from high light treated plants, (6) PSI preparation , (7) Pisum sativum BBY particles were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-PsbZ (AS06 115, 1:1000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent, using a Fuji LAS-3000 CCD (240s, standard sensitivity). There is a strong cross-reaction between 20-30 kDa.

Additional information

Related products

Background

Background

The 6.5 kDa PSII subunit PsbZ (ycf9 in Chlamydomonas) is coded by an open reading frame that is ubiquitously present in chloroplast and cyanobacterial genomes. PsbZ seems to control the interaction of PSII cores with the light-harvesting antenna involving changes in protein phosphorylation within PSII units, the deepoxidation state of xanthophylls, and the kinetics and amplitude of nonphotochemical quenching (NPQ).

Product citations

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