ACO1 | Aconitase

355 €

AS09 521 | Clonality:Polyclonal | Host:Rabbit | Reactivity: Arabidopsis thaliana, Solanum lycopersicum


21 st
Item No:
AS09 521

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product information

Aconitase is a single subunit enzyme of the tricarboxylic acid cycle (or Krebs cycle) in the mitochondria. A cytosolic isoform is also part of the glyoxylate cycle. Aconitase catalyzes the dehydration / hydration of citrate to iso-citrate, via cis-aconitate as an intermediate. The reaction is facilitated by an iron-sulphur cluster in the active site of the enzyme. The iron-sulphur cluster is somewhat unstable, especially under oxidative stress, and loss of the cofactor leads to degradation of the protein.Alternative names: ACO, citrate hydro-lyase 1,2,3


Arabidopsis ACO1 (AT4G35830, Q42560), codon 120 – 898 (C-terminus), was cloned in fusion with a N-terminal 6xHis tag, and over-expressed in E. coli. All recombinant protein accumulated in inclusion bodies, which were purified by centrifugation and solubilised in 6 M guanidine-HCl. The protein was refolded by dilution in 100 mM Tris-HCl 8.5, 10% (v/v) glycerol, 2 mM dithiothreitol, and concentrated prior to immunisation.

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
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Additional information

Arabidopsis expresses three highly similar aconitase isozymes (ACO1/ AT4G35830, ACO2/AT4G26970 and ACO3/AT2G05710), of which ACO1 is the cytosolic isoform, while ACO2 and ACO3 are predominantly located in the mitochondria (Arnaud et al 2007, Bernard et al 2009). The combined abundance and activity of the mitochondrial aconitases is about 3 times higher than the cytosolic pool (Bernard et al 2009).The Arabidopsis isoforms are more similar in amino acid sequence to mammalian iron-regulatory proteins (IRP-1) than to the mammalian and yeast mitochondrial aconitases.

application information
Recommended dilution 1 : 5 000 -1 : 10 000 (WB). At higher concentrations the antibody binds aspecifically resulting in non-specific signals around 60 kDa, including Rubisco subunits.
Expected | apparent MW

98 kDa. Note that ACO1, ACO2 and ACO3 cannot be distinguished in size by standard SDS-PAGE.

Confirmed reactivity Arabidopsis thaliana ACO1,ACO2 and ACO3 isoforms, Solanum lycopersicum
Predicted reactivity Cucurbita maxima, Nicotiana tabacum, Ricinus communis, Solanum tuberosum, Vitis vinifera, Oryza sativa, Zea mays, Picea sitcHensis, Populus trichocarpa
Not reactive in

Chlamydomonas reinhardii

Additional information

The antibody recognises all three Arabidopsis aconitase isoforms (ACO1, ACO2 and ACO3, see Bernard et al 2009). Possible differences in affinity have not been precisely quantified. Sensitivity threshold is between 2 and 10 ng for WB / ECL (see figure). Antibodies will recognize aconitase isoforms in denaturing and native gel electrophoresis.

Selected references Rurek et al. (2015). Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, organellar translation and ultrastructure. Biochim Biophys Acta. 2015 Jan 21. pii: S0005-2728(15)00016-X. doi: 10.1016/j.bbabio.2015.01.005.
Setién et al. (2014). Root phosphoenolpyruvate carboxylase and NAD-malic enzymes activity increase the ammonium-assimilating capacity in tomato. J Plant Physiol. 171:49-63.
Birke et al. (2012). Cysteine biosynthesis, in concert with a novel mechanism, contributes to sulfide detoxification in mitochondria of Arabidopsis thaliana. Biochem J. May 2, ahead of print.
Bernard et al. (2009). An allelic mutant series of ATM3 reveals its key role in the biogenesis of cytosolic iron-sulfur proteins in Arabidopsis. Plant Physiol. 151:590-602.

application example

Western blot analysis of 1) 5 ng purified 6xHis-AtACO1 (Δ119, 87 kDa); 2) 2 ng 6xHis-AtACO1; 3) Total protein (15 μg) from Arabidopsis thaliana leaves were extracted with 2 volumes 50 mM Tris-HCl pH 8.0, 5% (v/v) glycerol, 1% (w/v) sodium dodecyl sulphate, 10 mM NaEDTA, 1 mM phenylmethanesulfonyl fluoride; 4) 15 µg of purified mitochondria from Arabidopsis thaliana cell culture, 5) 15 µg of protein from Arabidopsis thaliana chloroplasts

Proteins were separated by SDS-PAGE and blotted onto nitrocellulose (Whatman Protran BA 83, 0.2 μm). Blots were blocked in Tris-buffered saline (TBS) with 0.1% (v/v) Tween 20 and 5% (w/v) dried skimmed milk for 1 h at room temperature, and incubated with anti-AtACO1 antibodies diluted 1:10,000 in fresh block solution (10 mL per 8 x 6 cm blot) for 2 h at room temperature. The blot was washed 3 times with block solution, then incubated with horse-radish peroxidase conjugated anti-rabbit IgG antibodies, diluted 1: 5,000 in block solution, for 45 minutes. The blot was washed 2 times with block solution and 2 times with TBS-Tween. The signal was developed with standard ECL reagents and Kodak X-Omat LS film.

Note: as visible in lane 2, detection of recombinant AtACO1 falls below 2 ng of recombinant protein.


  western blot detection using anti-aconitase antibodies

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