Cat | Catalase (peroxisomal marker) (100 ĩl)
AS09 501-100 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. napus, Ho. vulgare, N. bentamina, N.tabacum, O.sativa, Z.mays
|Recommended dilution||1 : 1000 (WB)|
|Expected | apparent MW||
57 | 55 kDa
|Confirmed reactivity||Arabidopsis thaliana, Brassica oleracea, Hordeum vulgare, Nicotiana bentamina, Nicotiana tabacum, Oryza sativa, Solanum lycopersicum, Spinacia oleracea, Zea mays, Vitis vinifera|
|Predicted reactivity||Cucumis sativus, Glycine max, Gossypium mexicanum, Populus jackii|
|Not reactive in||
To obtain reactivity with Solanum lycopersicum urea gel needs to be apply. Please, contact us for more details.
|Selected references||Kataya et al. (2015). Protein Phosphatase 2A Holoenzyme Is Targeted to Peroxisomes by Piggybacking and Positively Affects Peroxisomal β-Oxidation. Plant Physiol. 2015 Feb;167(2):493-506. doi: 10.1104/pp.114.254409.
Pilati et al. (2014). The onset of grapevine berry ripening is characterized by ROS accumulation and lipoxygenase-mediated membrane peroxidation in the skin. BMC Plant Biol. 2014 Apr 2;14(1):87.
Blume et al. (2013).A possible role for the chloroplast pyruvate dehydrogenase complex in plant glycolate and glyoxylate metabolism. Phytochemistry Aug2.
Li et al. (2013). LESION SIMULATING DISEASE1 Interacts with Catalases to Regulate Hypersensitive Cell Death in Arabidopsis. Plant Physiol. Aug 19.
Marok et al. (2013). A drought-sensitive barley variety displays oxidative stress and strongly increased contents in low-molecular weight antioxidant compounds during water deficit compared to a tolerant variety. J. Plant Physioology, Feb 8.
10 µg of total protein from Arabidopsis thaliana Col0 (1), Cat2-(Col0) (2), Ler0 (3), Cat2-(Ler0) (4), Zea mays (5), Oryza sativa (6), Brassica oleracea (7), Nicotiana bentamina (8) were extracted with 60mM Tris pH 6.9, 10mM DTT, 20% glycerol, 1mm PMSF were separated on 12.5% SDS-PAGE and blotted 1h to PVDF. Blot was blocked with 3% skim milk in PBS+0.05% Tween20 for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation in the same buffer. The antibody solution was decanted and the blot was rinsed briefly three times, then washed 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, Agrisera, AS09 602) diluted to 1:50 000 in 3% skim milk in PBS+0.05% Tween20 for 1h at RT with agitation. The blot was washed as above and developed for 1 min with Western Lightning Plus-ECL ( PerkinElmer )according to the manufacturers instructions. Exposure time was 5min. in ChemiDoc XRS+ (Biorad ).
Courtesy of Brigitte van de Cotte, Gent University, Belgium
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