COXII | Cytochrome oxidase subunit II (affinity purified)

AS04 053A | Clonality: Polyclonal | Host: Rabbit | Reactivity: eudicots, monocots, Physcomitrella patens | Cellular [compartment marker] of mitochondrial inner membrane

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Background		Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Alternative name: cytochrome c oxidase subunit 2
Immunogen		KLH-conugated synthetic peptide fully conserved in all available protein sequences from eudicots includingArabidopsis thaliana AtmG00160, monocots including Oryza sativa P04373 and Physcomitrella patens Q1XGA9
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Affinity purified serum
Format		Lyophilized in PBS pH 7.4
Quantity		50 µg
Reconstitution		For reconstitution add 50 µl of sterile water.
Storage		Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		Blue Native-PAGE (BN-PAGE), Western blot (WB)
Related products		AS04 052 anti-COXII hen antibody AS04 053A-200 COXII \| cytochrome oxidase subunit II AS04 053PRE \| COXII \| cytochrome oxidase subunit II, pre-immune serum AS04 053P COXII \| cytochrome oxidase subunit II \| Blocking peptide AS04 052 COXII \| cytochrome oxidase subunit II Antibodies to other mitochondrial proteins Recommended secondary antibody Plant protein extraction buffer Secondary antibodies
Additional information		Cellular [compartment marker] of mitochondrial inner membrane

application information
Recommended dilution		1 : 1000 (BN-PAGE), 1 : 1000 (WB)
Expected \| apparent MW		29.4 \| 30 kDa (for Arabidopsis thaliana)
Confirmed reactivity		Arabidopsis thaliana (leaf extract and isolated mitochondria), Betula nana, Brassica napus, Brassica oleracea, Cicer aretinum, Cucumis melo, Erophorum vaginatum, Hordeum vulgare, Lilium longifolorum, Nicotiana tabacum, Picea abies, Plantago major, Plantago euryphylla, Silene uniflora, Silene dioica, Physcomitrella patens, Triticum aestivum, Triticum durum Desf. , Zea mays, Vicia faba, Quercus rubra
Predicted reactivity		Cucumis melo, Glycine max, Oryza sativa, Physcomitrella patens, Pisum sativum, Triticum aestivum
Not reactive in		Saccharina japonica
Additional information		Antibody detects COXII protein most optimally in membrane fractions. The signal is weak in a in total protein extract. Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.
Selected references		Dai et al. (2018). Maize Dek37 Encodes a P-Type PPR Protein That Affects Cis-splicing of Mitochondrial nad2 Intron 1 and Seed Development. Genetics. 2018 Jan 4. pii: genetics.300602.2017. doi: 10.1534/genetics.117.300602. Nagel et al. (2017). Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3. Proc Natl Acad Sci U S A. 2017 Aug 7. pii: 201710866. doi: 10.1073/pnas.1710866114. Garmash et al. (2017). Expression profiles of genes for mitochondrial respiratory energy-dissipating systems and antioxidant enzymes in wheat leaves during de-etiolation. J Plant Physiol. 2017 Aug;215:110-121. doi: 10.1016/j.jplph.2017.05.023. Weißenberger et al. (2017). The PPR protein SLOW GROWTH 4 is involved in editing of nad4 and affects the splicing of nad2 intron 1. Plant Mol Biol. 2017 Mar;93(4-5):355-368. doi: 10.1007/s11103-016-0566-4. Cai et al. (2017). Emp10 encodes a mitochondrial PPR protein that affects the cis-splicing of nad2 intron 1 and seed development in maize. Plant J. 2017 Mar 27. doi: 10.1111/tpj.13551. Schimmeyer et al. (2016). L-Galactono-1,4-lactone dehydrogenase is an assembly factor of the membrane arm of mitochondrial complex I in Arabidopsis. Plant Mol Biol. 2016 Jan;90(1-2):117-26. doi: 10.1007/s11103-015-0400-4. Epub 2015 Oct 31. Li et al. (2016). Characterization of a novel β-barrel protein (AtOM47) from the mitochondrial outer membrane of Arabidopsis thaliana. J Exp Bot. 2016 Nov;67(21):6061-6075. Epub 2016 Oct 6. Pavlovič et al. (2016). Light-induced gradual activation of photosystem II in dark-grown Norway spruce seedlings. Biochim Biophys Acta. 2016 Feb 18. pii: S0005-2728(16)30028-7. doi: 10.1016/j.bbabio.2016.02.009.

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