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AGO set
Tag Antibody Set 

FtsH10 | ATP-dependent zinc metalloprotease FtsH10 (mitochondrial)

AS07 251 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

FtsH10 | ATP-dependent zinc metalloprotease FtsH10 (mitochondrial) in the group Antibodies for Plant/Algal  / Mitochondria | Respiration at Agrisera AB (Antibodies for research) (AS07 251)

PRODUCT INFORMATION IN PDF

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Datasheet Product citations Add review

Product Information

Immunogen

KLH-conjugated peptide located near C-terminus chosen from sequence of Arabidopsis thaliana FtsH10 Q8VZI8, At1g07510

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Blue Native PAGE (BN-PAGE), Immunoprecipitation (IP)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

84 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity

Arabidopsis thaliana

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example


western blot detection using anti-FtsH10 antibodies

 

Mitochondrial preapration from Arabidopsis thaliana mitochondria was separated on 10% acrilamide gel and electrophoresis prepared according to Schägger and von Jagov (Anl. Biochem., 1987, 166:368-379). After running the gel, proteins were transferred to nitrocellulose membrane using wet transfer (0.22% CAPS, pH 11). Transfer was checked by Ponceau S staining. Blot was destained by several quick washings in distilled water and 1 washing in 1X TBS (10 mM T pH 7.5, 150 mM NaCl) (10-15 min.).Blot was blocked by 1.5 hour in 5% milk in TBST (1X TBS, 0,1 20) After blocking blot was washed quickly twice in TBST and incubated 2 hours with primary antibody (dilution 1: 1000 TBST (dilution 1:1000). Washing: two quick washings in TBST and 3 x 10 min. washings in TBST. Then blot was incubated 45-60 min. with a secondary anti-rabbit antibodies conjugated to peroxidase (dilution 1:10000) in TBST. Washing: as above. After washing blot was incubated 1-2 min. in ECL solution and exposed to Kodak autoradiography film. Exposure time was 15-60 seconds.

Mitochondria were isolated as described by Urantowka et al. (Plant Mol Biol, 2005, 59:239-52). Mitochondrial pellets were suspended in 1X Laemmli buffer (5% beta-mercaptoetanol, 3.7% glycerol, 1.1% SDS, 23 mM Tris-HCl pH 6.8, 0.01% bromophenol blue), heated (95 ºC, 5 min.) and centrifuged (13000 rpm, 1 min.).

Courtesy Dr. J. Piechota, University of Wroclaw, Poland

 

Additional information

Additional information

Blue-native (2D BN/SDS-PAGE) methodology has been described in Piechota et al. 2010

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AS16 3930 | anti-FtsH1 + FtsH5 | ATP-dependent zinc metalloprotease FtsH1 + FtsH5 (chloroplastic)
AS16 3929 | anti-FtsH2 + FtsH8 | ATP-dependent zinc metalloprotease FtsH2 + FtsH8 (chloroplastic)
AS07 204 | anti-FtsH3 + FtsH10 | ATP-dependent zinc metalloprotease FtsH3 + FtsH10 (mitochondrial)
AS07 205 | anti-FtsH4 | ATP-dependent zinc metalloprotease FtsH4 (mitochondrial)
AS05 094A | anti-FtsH6 | ATP-dependent zinc metalloprotease FtsH6 (chloroplastic)
AS06 130 | anti-FtsH9 | ATP-dependent zinc metalloprotease FtsH9 (chloroplastic)

Antibodies to other proteins involved in photosynthesis

Plant protein extraction buffer

Secondary antibodies

Background

Background

One of the several classes of mitochondrial proteases is membrane bound, ATP dependent FtsH protease. Their function is very important for the control of protein quality and quantity by degradation of unassembled subunits. FtsH10 is localized in mitochondria. Alternative names: cell division protease ftsH homolog 10, mitochondrial, AtFtsH10

Product citations

Selected references Kolodziejczak et al. (2018). m-AAA Complexes Are Not Crucial for the Survival of Arabidopsis Under Optimal Growth Conditions Despite Their Importance for Mitochondrial Translation. Plant Cell Physiol. 2018 May 1;59(5):1006-1016. doi: 10.1093/pcp/pcy041.
Piechota et al. (2015). Unraveling the functions of type II-prohibitins in Arabidopsis mitochondria. Plant Mol Biol. 2015 Apr 21.
Kwasniak et al. (2013). Silencing of the Nuclear RPS10 Gene Encoding Mitochondrial Ribosomal Protein Alters Translation in Arabidiopsis Mitochondria. Plant Cell, May 30.
Quesada et al. (2011). Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development. Plant J.