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AGO set
Tag Antibody Set 

GLDH | Galactono-1,4 lactone dehydrogenase

AS06 182  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity:  H. vulgare, G. max,  A. sativa, Helianthus annuus, Z.mays

GLDH | Galactono-1,4 lactone dehydrogenase in the group Antibodies for Plant/Algal  / Mitochondria | Respiration at Agrisera AB (Antibodies for research) (AS06 182)

PRODUCT INFORMATION IN PDF

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355 €

Datasheet Product citations Add review

Product Information

Immunogen

Recombinant C-terminal of Zea mays GLDH, UniProt: C0HFL3

Host Rabbit
Clonality Polyclonal
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

68 kDa

Reactivity

Confirmed reactivity Avena sativa, Glycine max, Hordeum vulgare, Helianthus annuus, Zea mays
Predicted reactivity

Arabidopsis thaliana, Oryza sativa, Zostera marina

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

Total IgG concentration is 6.8 μg/μl

Mitochondrial, membrane or meristematic fractions were shown to be richer in GLDH expression

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Background

Background

Galactono-1,4-lactone dehydrogenase (GLDH) is the enzyme which catalyses last step of ascorbic acid (AA)  synthesis in the mitochondria of plant cells. Synonyme: GaLDH

Product citations

Selected references Schimmeyer et al. (2016). L-Galactono-1,4-lactone dehydrogenase is an assembly factor of the membrane arm of mitochondrial complex I in Arabidopsis. Plant Mol Biol. 2016 Jan;90(1-2):117-26. doi: 10.1007/s11103-015-0400-4. Epub 2015 Oct 31.
Ostaszewska-Bugajska et al. (2016). Changes in the OXPHOS system in leaf and root mitochondria of Arabidopsis thaliana subjected to long-term sulphur deficiency. Acta Physiologiae Plantarum 38:141.
Bartoli et al. (2005). Ascorbate content in wheat leaves is not determined by maximal L-galactono-1, 4-lactone dehydrogenase (GalLDH) activity under drought stress. Plant Cell Environ 28:1073-1081.