NAD3 | NADH-ubiquinone oxidoreductase chain 3

339 €
AS16 3831 | Clonality: Polyclonal | Host: Rabbit | ractivity: Oryza sativa


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Item No:
AS16 3831

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product information
Background NAD3 (NADH dehydrogenase subunit 3) is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). It functions in the transfer of electrons from NADH to the respiratory chain.
Immunogen KLH-conjugated synthetic peptide chosen from Oryza sativa NAD3 protein sequence UniProt: Q7GHI8; LOC_Osm1g00280.1
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 2 x 50 µl
Reconstitution For reconstitution add 50 ĩl of sterile water to each tube.
Storage Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles.
Tested applications Western blot (WB)
Related products AS15 2926 | Anti-NAD6 | NADH-ubiquinone oxidoreductase chain 6, rabbit antibodies
collection of antibodies to mitochondrial proteins
Additional information
application information
Recommended dilution 1: 1000 (WB)
Expected | apparent MW

17 kDa

Confirmed reactivity Oryza sativa
Predicted reactivity
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references To be added when available, antibody released in April 2016.

Application example

western blot using anti-NAD3 rabbit antibodies

50-100 µg of total protein from Oryza sativa flag leaf, roots or fringe extracted with protein extraction reagent were separated on 15% SDS-PAGE and blotted 1h to PVDF using wet transfer. Blots were blocked with 1×TBST+5% Nonfat dry milk for 1h at 37°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 -1:1000 in 1×TBST+5% Nonfat dry milk for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min. and 3 times for 5 min. in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG HRP/conjugated) diluted to 1:15 000 in 1x TBST + 5% Nonfat dry milk for 2h at RT with agitation. The blot was washed as above and developed for 5 min. with ECL according to the manufacturer's instructions. Exposure time was 10 seconds.

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