NAD6 | NADH-ubiquinone oxidoreductase chain 6

AS15 2926  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

NAD6 | NADH-ubiquinone oxidoreductase chain 6 in the group Plant/Algal Antibodies / Mitochondria | Respiration at Agrisera AB (Antibodies for research) (AS15 2926)


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product information
Background NAD6 (NADH-ubiquinone oxidoreductase chain 6) is involved in cellular respiration, oxidation-reduction process. Protein is a part of mitochondrial respiratory chain complex I and displays NADH dehydrogenase activity.
Immunogen KLH-conjuagted peptide derived from Arabdiopsis thaliana sequence, UniProt: P60497, TAIR:ATMG00270
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

antibodies to other mitochondrial proteins
recommended secondary antibody

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

23.4 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Asclepias syriaca, Batis maritima, Beta vulgaris subsp. maritima, Beta macrocarpa, Bischofia javanica, Brassica carinata, Brassica napus, Brassica oleracea, Brassica rapa, Brexia madagascariensis , California macrophylla, Carallia brachiata, Caryocar glabrum, Celastrus orbiculatus, Chrysobalanus icaco, Citrullus lanatus, Couepia guianensis, Ctenolophon englerianus , Cucurbita pepo, Dasiphora fruticosa, Dissiliaria muelleri, Elaeodendron orientale, Eruca sativa, Fragaria vesca, Geranium sanguineum, Goupia glabra, Gymnosporia senegalensis, Hevea brasiliensis, Hirtella physophora, Humiria balsamifera, Hypseocharis bilobata, Licania heteromorpha, Lupinus luteus, Lupinus mutabilis, Malus domestica , Monsonia emarginata, Neoscortechinia kingii, Parinari campestris, Phaseolus angularis, Poliothyrsis sinensis, Prunus persica, Pyrus x bretschneideri, Raphanus sativus, Reinwardtia indica, Rhazya stricta, Trigonia nivea, Silene latifolia, Silene noctiflora, Sorghum bicolor, Utricularia gibba, Zea mays, Vigna radiata, Vicia faba
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information There is a cross-reaction with a thylakoid protein, therefore this antibody may not be usable for western blots of total leaf extracts. 
Selected references to be added when available, antibody released in December 2015

application information

western blot using plant anti-NAD6 antibodies

12 µg of Arabidopsis thaliana leaf mitochondrial protein (Mini-protean 3, BioRad) denatured with 2x Laemmli solubilisation buffer at 65ºC for 5-10 min were separated on 16 % SDS-PAGE and blotted 45 min to PVDF using semi-dry transfer. Blots were blocked with commercial blocker from Roche at 1:10 dilution for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight at 4ºC with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, Agrisera AS09 602) diluted to 1: 15 000 in for 1h at RT with agitation. The blot was washed as above and developed for min with Clarity Western ECL substrate from BioRad. Exposure time was 1 min for the blots probed with the purified antibody. Green arrow is pointing out at contamination band present in a mutant.

Courtesy of Dr. Catherine Colas des Francs-Small, UWA, Australia

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