UCP | Uncoupling protein

AS12 1850 | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, G.max, S. lycopersicum, T. aestivum, V. faba

UCP | Uncoupling protein in the group Plant/Algal Antibodies / Mitochondria | Respiration at Agrisera AB (Antibodies for research) (AS12 1850)


381 €

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product information

UCP (uncoupling protein) is an inner membrane mitochondrial protein that can dissipate the proton gradien before it can be used to provide the energy for oxidative phosphorylation. Synonymes: AtUCP, Uncoupling protein 2.


KLH-conjugated synthetic peptide derived from known UCP protein sequences, including UCP1 (AT3G54110) and UCP2 (AT5G58970) of Arabidopsis thaliana

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized in PBS pH 7.4
Quantity 200 µg
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

antibodies to other plant mitochondrial proteins

Plant and algal protein extraction buffer

Secondary antibodies

Additional information

Peptide used to elicit this antibody is conserved in both isoforms, UCP1 and UCP2 of Arabidopsis thaliana.

application information
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW

32 kDa

Confirmed reactivity Arabidopsis thaliana, Splanum lycopersicum, Triticum aestivum, Vicia faba (protoplasts)
Predicted reactivity Citrus sinensis, Dracunulus vulgaris, Glycine max, Litchi chinensis, Medicago tribuloides, Oryza sativa, Populus trichocarpa, Ricinus communis, Saccharum officinarium (sugarcane), Triticum aestivum
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references Garmash et al. (2017). Expression profiles of genes for mitochondrial respiratory energy-dissipating systems and antioxidant enzymes in wheat leaves during de-etiolation. J Plant Physiol. 2017 Aug;215:110-121. doi: 10.1016/j.jplph.2017.05.023.
Florez-Sarasa et al. (2016). Impaired cyclic electron flow around Photosystem I disturbs high-light respiratory metabolism. Plant Physiol. 2016 Oct 19. pii: pp.01025.2016.
Liu et al. (2015). Silencing of mitochondrial uncoupling protein gene aggravates chilling stress by altering mitochondrial respiration and electron transport in tomato. Acta Physiologiae Plantarum November 2015, 37:223.
Long et al. (2015). Contributions of photosynthetic and non-photosynthetic cell types to leaf respiration in Vicia faba L. and their responses to growth temperature. Plant Cell Environ. 2015 Apr 1. doi: 10.1111/pce.12544.
Grabelnych et al. (2014). Mitochondrial Energy Dissipating Systems (Alternative Oxidase, Uncoupling Proteins, and External NADH Dehydrogenase) Are Involved in Development of Frost Resistance of Winter Wheat Seedlings. ISSN 0006 2979, Biochemistry (Moscow), 2014, Vol. 79, No. 6, pp. 506 519. © Pleiades Publishing, Ltd., 2014.
Barreto et al. (2014). Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response. BMC Plant Biol. 2014 May 28;14(1):144.

application example


western blot using anti-plant UCP antibody

10 μg of mitochondrial fraction from Arabidopsis thaliana and 25 μg of Arabidopsis thaliana leaf extract were separated on 10% gel and blotted on nitrocellulose membrane using wet transfer (0.22% CAPS, pH 11). Filters where blocked (1.5h) in 5% milk in TBST (1X TBS, 0,1% Tween 20), incubated with 1: 5000 anti-VDAC1 antibodies (2h in TBST) followed by incubation with 1: 10 000 secondary anti-rabbit (1h) HRP-coupled antibodies from Agrisera, AS09 602 and visualized with standard ECL on Kodak autoradiography film for 15-60 s. Mitochondria were isolated as described by Urantowka et al. (Plant Mol Biol, 2005, 59:239-52). Mitochondrial pellets were suspended in 1X Laemmli buffer (5% beta-mercaptoetanol, 3.7% glycerol, 1.1% SDS, 23 mM Tris- HCl pH 6.8, 0.01% bromophenol blue), heated (95°C, 5 min.) and centrifuged (13 000rpm, 1 min.). Leaf extracts were prepared as described by Martinez-Garcia et al. (Plant J., 1999, 20:251-7).
Courtesy Dr. Janusz Piechota, Wrocław University, Poland

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