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Tag Antibody Set 

VDAC1 | Voltage-dependent anion-selective channel protein 1, HRP-conjugated (40 ĩg)

AS07 212-HRP  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A.thaliana, di and monocots | Cellular [compartment marker] of mitochondrial outer membrane

VDAC1 | Voltage-dependent anion-selective channel protein 1, HRP-conjugated (40 ĩg) in the group Antibodies for Plant/Algal  / Mitochondria | Respiration at Agrisera AB (Antibodies for research) (AS07 212-HRP)

PRODUCT INFORMATION IN PDF

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417 €

Datasheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated peptide conserved in all known higher plant VDAC1 proteins including Arabidopsis thaliana with the locus name: At3g01280

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Liquid in PBS pH 7.4, conjugated to HRP
Quantity 40 µg
Storage Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Tested applications Blue-native PAGE (2D BN/SDS-PAGE), Immunolocalization (IL), Western blot (WB)
Recommended dilution 1 : 500 (IL), 1 : 5000, 2-30 µg protein/lane (WB)
Expected | apparent MW

29 kDa (for Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Beta vulgaris, Brassica oleracea var. botrytis, Oryza sativa, Papaver sp. pollen tubes (IL), Spinacia oleracea, Physcomitrella patens
Predicted reactivity

Arabidopsis alpina, Aundo donax, Brachypodium distachyon, Brassica campestris, Brassica napus, Brassica rapa subsp. pekinensis,  Capsella rubella, Citrus clementina, Citrus sinensis, Eutrema salsugineum, Glycine max, Glycine soja, Gossypium arboreum, Hoedum vulgare var. distichum, Jatropha curcas, Medicago truncatula, Mesembryanthemum crystallinum,  Morus notabilis, Nicotiana tabacum, Phaseolus coccineus, Phaseolus vulgaris, Pisum sativum, Plantago major, Prunus persicaRicinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Vitis vinifera, Zea mays

Not reactive in

Chlamydomonas reinhardtii, Glycine max, Zea mays, diatoms, Saccharomyces cerevisiae

Application examples

Additional information

Additional information Cellular [compartment marker] of mitochondrial outer membrane

Amount of mitochondrial fraction detected by anti-VDAC1 antibody was from 2-10 µg.

Immunolocalization method description and images are available here

Blue-native (2D BN/SDS-PAGE) methodology is described in Piechota et al. 2010

Related products

Related products AS07 212 | Anti-VDAC1 | Voltage-dependent anion-selective channel protein 1, rabbit antibody
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collection of antibodies to other mitochondrial proteins

Background

Background

VDAC1 protein (called also Synonymes: At3g01280, outer mitochondrial membrane protein porin 1, T22N4_9, T22N4.9, VDAC 1, Voltage-dependent anion-selective channel protein 1, voltage-gated ion-selective channel) forms a channel through the cell membrane for diffusion of small hydrophilic molecules. Evolutionary origin of VDAC protein is not clear and their structure and properties are quite different making those proteins only conceptually like porins (Clausen et al. 2004).

Product citations

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