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PLD | Phospholipase D (affinity purified)

AS09 558 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Brassica oleracea | Note: Limited stock

PLD | Phospholipase D (affinity purified) in the group Antibodies for Plant/Algal  / Developmental Biology / Lipid metabolism at Agrisera AB (Antibodies for research) (AS09 558)

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440
How to cite this product:
Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

phospholipase D isolated and purified from Brassica oleracea

Host Rabbit
Clonality Polyclonal
Purity Affinity purified IgG
Format Lyophilized in phosphate buffer saline
Quantity 1 mg
Reconstitution For reconstitution add 0.5 ml of sterile destilled water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 100-1 : 1000 (ELISA), (IF), (IHC), (WB)
Expected | apparent MW

92 kDa

Reactivity

Confirmed reactivity Brassica oleracea
Predicted reactivity Arabidopsis thaliana, Cucumis melo, Ricinus communis, Populus balsamifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.

Related products

Related products

AS09 556 | anti-PLD phospholipase D

AS09 557 | anti-PLD phospholipase D (biotinylated)

Plant protein extraction buffer

Secondary antibodies

Background

Background

Phospholipase D (EC=3.1.4.4) is an enzyme involved in lipid degradation by hydrolyzing glycerol phospholipids. Involved in many cellular process including phytohormone action, membrane deterioration, senescence and more. Alternative name: phosphatidylcholine phosphatido-hydrolase.

Product citations

Selected references

to be added when available