PLD | phospholipase D, Biotin conjugated

AS09 557 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Brassica oleracea

PLD | phospholipase D, Biotin conjugated

Product Information

Immunogen Phospholipase D isolated and purified from Brassica oleracea

Host Rabbit

Clonality Polyclonal

Purity Purified IgG in PBS.

Format Lyophilized

Quantity 10 mg

Reconstitution For reconstitution add 0,5 ml of sterile destilled water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 1000-1 : 20 000 (ELISA), (IF), (IHC), (WB)

Expected | apparent MW

92 kDa

Reactivity

Confirmed reactivity Brassica oleracea

Predicted reactivity Arabidopsis thaliana, Cucumis melo, Ricinus communis, Populus balsamifera
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Antibody is labelled with biotin using N-hydroxysuccinimidobiotin, Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition

Biotin/IgG protein molar ration is approximately 6,2, No foreign proteins are added

Background

Phospholipase D (EC=3.1.4.4) is an enzyme involved in lipid degradation by hydrolyzing glycerol phospholipids. Involved in many cellular process including phytohormone action, membrane deterioration, senescence and more.

Alternative name: phosphatidylcholine phosphatido-hydrolase.

Product citations

Related products: PLD | phospholipase D, Biotin conjugated

Ficain | ficin, Biotin conjugated

AS09 551 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Ficus glabRata (fig)

401 €

PLD | Phospholipase D (affinity purified)

AS09 558 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Brassica oleracea | Note: Limited st...

546 €

PLD | Phospholipase D

AS09 556 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Brassica oleracea

248 €

PPI | papain, Biotin conjugated

AS09 546 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Carica papaya

401 €

calculated \| apparent molecular mass [kDa]:	92 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	Phospholipase D isolated and purified from Brassica oleracea
Purity:	Purified IgG in PBS.
Quantity:	10 mg
recommended dilution:	1 : 1000-1 : 20 000 (ELISA), (IF), (IHC), (WB)
Reconstitution:	For reconstitution add 0,5 ml of sterile destilled water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
additional information:	Antibody is labelled with biotin using N-hydroxysuccinimidobiotin, Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
additional information (application):	Biotin/IgG protein molar ration is approximately 6,2, No foreign proteins are added
Confirmed reactivity:	Brassica oleracea
predicted reactivity:	Arabidopsis thaliana, Cucumis melo, Ricinus communis, Populus balsamifera Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
background:	Phospholipase D (EC=3.1.4.4) is an enzyme involved in lipid degradation by hydrolyzing glycerol phospholipids. Involved in many cellular process including phytohormone action, membrane deterioration, senescence and more. Alternative name: phosphatidylcholine phosphatido-hydrolase.