PLD | phospholipase D, Biotin conjugated

AS09 557 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Brassica oleracea

Product Information

Immunogen

phospholipase D isolated and purified from Brassica oleracea

Host Rabbit

Clonality Polyclonal

Purity Purified IgG

Format Lyophilized in phosphate buffer saline

Quantity 1 ml

Reconstitution For reconstitution add 0.5 ml of sterile destilled water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 1000-1 : 20 000 (ELISA), (IF), (IHC), (WB)

Expected | apparent MW

92 kDa

Reactivity

Confirmed reactivity Brassica oleracea

Predicted reactivity Arabidopsis thaliana, Cucumis melo, Ricinus communis, Populus balsamifera

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.

Related products

AS09 556 | anti-PLD phospholipase D

AS09 558 | anti-PLD phospholipase D (affinity purified)

Plant protein extraction buffer

Background

Phospholipase D (EC=3.1.4.4) is an enzyme involved in lipid degradation by hydrolyzing glycerol phospholipids. Involved in many cellular process including phytohormone action, membrane deterioration, senescence and more. Alternative name: phosphatidylcholine phosphatido-hydrolase.

Product citations

Selected references

to be added when available