PLD | phospholipase D, Biotin conjugated

AS09 557 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Brassica oleracea

Product Information

Immunogen Phospholipase D isolated and purified from Brassica oleracea

Host Rabbit

Clonality Polyclonal

Purity Purified IgG

Format Lyophilized in phosphate buffer saline

Quantity 10 mg

Reconstitution For reconstitution add 0,5 ml of sterile destilled water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes

Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 1000-1 : 20 000 (ELISA), (IF), (IHC), (WB)

Expected | apparent MW

92 kDa

Reactivity

Confirmed reactivity Brassica oleracea

Predicted reactivity Arabidopsis thaliana, Cucumis melo, Ricinus communis, Populus balsamifera
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Antibody is labelled with biotin using N-hydroxysuccinimidobiotin, Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition

Biotin/IgG protein molar ration is approximately 6,2, No foreign proteins are added

Related products

AS09 556 | Anti-PLD phospholipase D, rabbit antibodies

AS09 558 | Anti-PLD phospholipase D (affinity purified), rabbit antibodies

Plant protein extraction buffer

Background

Phospholipase D (EC=3.1.4.4) is an enzyme involved in lipid degradation by hydrolyzing glycerol phospholipids. Involved in many cellular process including phytohormone action, membrane deterioration, senescence and more.

Alternative name: phosphatidylcholine phosphatido-hydrolase.

Product citations

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