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PLD | phospholipase D, Biotin conjugated

AS09 557 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Brassica oleracea

PLD | phospholipase D, Biotin conjugated in the group Antibodies Plant/Algal  / Developmental Biology / Lipid metabolism at Agrisera AB (Antibodies for research) (AS09 557)
PLD | phospholipase D, Biotin conjugated



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Product Information

Immunogen Phospholipase D isolated and purified from Brassica oleracea
Host Rabbit
Clonality Polyclonal
Purity Purified IgG in PBS.
Format Lyophilized
Quantity 10 mg
Reconstitution For reconstitution add 0,5 ml of sterile destilled water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 1000-1 : 20 000 (ELISA), (IF), (IHC), (WB)
Expected | apparent MW

92 kDa

Reactivity

Confirmed reactivity Brassica oleracea
Predicted reactivity Arabidopsis thaliana, Cucumis melo, Ricinus communis, Populus balsamifera
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Antibody is labelled with biotin using N-hydroxysuccinimidobiotin, Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
Biotin/IgG protein molar ration is approximately 6,2, No foreign proteins are added

Related products

Related products

AS09 556 | Anti-PLD phospholipase D, rabbit antibodies

AS09 558 | Anti-PLD phospholipase D (affinity purified), rabbit antibodies

Plant protein extraction buffer

Background

Background

Phospholipase D (EC=3.1.4.4) is an enzyme involved in lipid degradation by hydrolyzing glycerol phospholipids. Involved in many cellular process including phytohormone action, membrane deterioration, senescence and more.

Alternative name: phosphatidylcholine phosphatido-hydrolase.

Product citations

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