PLD | Phospholipase D

AS09 556 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Brassica oleracea

Product Information

Immunogen

phospholipase D isolated and purified from Brassica oleracea

Host Rabbit

Clonality Polyclonal

Purity Purified IgG in PBS.

Format Lyophilized

Quantity 10 mg

Reconstitution For reconstitution add 1 ml of sterile destilled water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 1000-1 : 7 000 (ELISA), (IF), (IHC), (WB)

Expected | apparent MW

92 kDa

Reactivity

Confirmed reactivity Brassica oleracea

Predicted reactivity Arabidopsis thaliana, Cucumis melo, Ricinus communis, Populus balsamifera, Zea mays
Species of your interest not listed? Contact us

Not reactive in Corynebacterium pseudotuberculosis

Application examples

Additional information

Additional information Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition

The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography

Background

Phospholipase D (EC=3.1.4.4) is an enzyme involved in lipid degradation by hydrolyzing glycerol phospholipids. Involved in many cellular process including phytohormone action, membrane deterioration, senescence and more.

Alternative name: phosphatidylcholine phosphatido-hydrolase.

Product citations

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Confirmed reactivity:	Brassica oleracea
predicted reactivity:	Arabidopsis thaliana, Cucumis melo, Ricinus communis, Populus balsamifera, Zea mays Species of your interest not listed? Contact us
not reactive in:	Corynebacterium pseudotuberculosis
calculated \| apparent molecular mass [kDa]:	92 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	phospholipase D isolated and purified from Brassica oleracea
Purity:	Purified IgG in PBS.
Quantity:	10 mg
recommended dilution:	1 : 1000-1 : 7 000 (ELISA), (IF), (IHC), (WB)
Reconstitution:	For reconstitution add 1 ml of sterile destilled water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
additional information:	Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
additional information (application):	The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography
background:	Phospholipase D (EC=3.1.4.4) is an enzyme involved in lipid degradation by hydrolyzing glycerol phospholipids. Involved in many cellular process including phytohormone action, membrane deterioration, senescence and more. Alternative name: phosphatidylcholine phosphatido-hydrolase.