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PLD | Phospholipase D

AS09 556 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Brassica oleracea

PLD | Phospholipase D in the group Antibodies Plant/Algal  / Developmental Biology / Lipid metabolism at Agrisera AB (Antibodies for research) (AS09 556)
PLD | Phospholipase D



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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

phospholipase D isolated and purified from Brassica oleracea

Host Rabbit
Clonality Polyclonal
Purity Purified IgG in PBS.
Format Lyophilized
Quantity 10 mg
Reconstitution For reconstitution add 1 ml of sterile destilled water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 1000-1 : 7 000 (ELISA), (IF), (IHC), (WB)
Expected | apparent MW

92 kDa

Reactivity

Confirmed reactivity Brassica oleracea
Predicted reactivity Arabidopsis thaliana, Cucumis melo, Ricinus communis, Populus balsamifera, Zea mays
Species of your interest not listed? Contact us
Not reactive in Corynebacterium pseudotuberculosis

Application examples

Additional information

Additional information Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography

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Background

Background

Phospholipase D (EC=3.1.4.4) is an enzyme involved in lipid degradation by hydrolyzing glycerol phospholipids. Involved in many cellular process including phytohormone action, membrane deterioration, senescence and more.

Alternative name: phosphatidylcholine phosphatido-hydrolase.

Product citations

Confirmed reactivity: Brassica oleracea
predicted reactivity: Arabidopsis thaliana, Cucumis melo, Ricinus communis, Populus balsamifera, Zea mays
Species of your interest not listed? Contact us
not reactive in: Corynebacterium pseudotuberculosis
calculated | apparent molecular mass [kDa]:

92 kDa

Clonality: Polyclonal
Format: Lyophilized
Host: Rabbit
immunogen:

phospholipase D isolated and purified from Brassica oleracea

Purity: Purified IgG in PBS.
Quantity: 10 mg
recommended dilution: 1 : 1000-1 : 7 000 (ELISA), (IF), (IHC), (WB)
Reconstitution: For reconstitution add 1 ml of sterile destilled water
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
additional information: Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
additional information (application): The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography
background:

Phospholipase D (EC=3.1.4.4) is an enzyme involved in lipid degradation by hydrolyzing glycerol phospholipids. Involved in many cellular process including phytohormone action, membrane deterioration, senescence and more.

Alternative name: phosphatidylcholine phosphatido-hydrolase.

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