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PLD | Phospholipase D

AS09 556 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Brassica oleracea

PLD | Phospholipase D in the group Antibodies for Plant/Algal  / Developmental Biology / Lipid metabolism at Agrisera AB (Antibodies for research) (AS09 556)

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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

phospholipase D isolated and purified from Brassica oleracea

Host Rabbit
Clonality Polyclonal
Purity Purified IgG
Format Lyophilized in phosphate buffer saline
Quantity 10 mg
Reconstitution For reconstitution add 1 ml of sterile destilled water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 1000-1 : 7 000 (ELISA), (IF), (IHC), (WB)
Expected | apparent MW

92 kDa

Reactivity

Confirmed reactivity Brassica oleracea
Predicted reactivity Arabidopsis thaliana, Cucumis melo, Ricinus communis, Populus balsamifera, Zea mays
Not reactive in Corynebacterium pseudotuberculosis

Application examples

Additional information

Additional information Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Related products

Related products

AS09 557 | anti-PLD phospholipase D (biotinylated), rabbit anitbody

AS09 558 | anti-PLD phospholipase D (affinity purified), rabbit antibody

AS12 2364 | anti-PLDA1/2 | phospholipase D alpha 1/2, rabbit antibody with broader species reactivity

Plant protein extraction buffer

Secondary antibodies

Background

Background

Phospholipase D (EC=3.1.4.4) is an enzyme involved in lipid degradation by hydrolyzing glycerol phospholipids. Involved in many cellular process including phytohormone action, membrane deterioration, senescence and more. Alternative name: phosphatidylcholine phosphatido-hydrolase.

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