PLD | Phospholipase D
AS09 556 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Brassica oleracea
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Product Information
Immunogen
phospholipase D isolated and purified from Brassica oleracea
Host
Rabbit
Clonality
Polyclonal
Purity
Purified IgG
Format
Lyophilized in phosphate buffer saline
Quantity
10 mg
Reconstitution
For reconstitution add 1 ml of sterile destilled water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 1000-1 : 7 000 (ELISA), (IF), (IHC), (WB)
Expected | apparent MW
92 kDa
Reactivity
Confirmed reactivity
Brassica oleracea
Predicted reactivity
Arabidopsis thaliana, Cucumis melo, Ricinus communis, Populus balsamifera, Zea mays
Not reactive in
Corynebacterium pseudotuberculosis
Application examples
Additional information
Additional information
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
Background
Background
Phospholipase D (EC=3.1.4.4) is an enzyme involved in lipid degradation by hydrolyzing glycerol phospholipids. Involved in many cellular process including phytohormone action, membrane deterioration, senescence and more. Alternative name: phosphatidylcholine phosphatido-hydrolase.
Product citations
Selected references
to be added when available
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