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AGO set
Tag Antibody Set 

PsaC | PSI-C core subunit of photosystem I (PsaC antibody + PsaC protein positive control )

AS04 042P | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, cyanobacteria

PsaC | PSI-C core subunit of photosystem I (PsaC antibody + PsaC protein positive control ) in the group Antibodies for Plant/Algal  / Global Antibodies at Agrisera AB (Antibodies for research) (AS04 042P)

PRODUCT INFORMATION IN PDF

Qty: 
360 €

Datasheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide conserved in all known PsaC proteins includingArabidopsis thaliana AtCg01060, Hordeum vulgare P69416, Oryza sativa P0C360, Chlamydomonas reinhardtii Q00914, Synechococcus elongatus Q31QV2

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl anti-PsaC antibody, 100 µl PsaC protein standard
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

9 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Chlamydomonas reinhardtii, Cyanophora paradoxa, Heterosigma akashiwo, Emiliania huxleyi, Euglena gracilis, Gonyaulax polyedra, Horderum vulgare, Micromonas pusilla, Porphyra sp. Spinacia oleracea, Synechococcus PCC 7942,  Thalassiosira pseudonan
Predicted reactivity

Algae, Cyanobacteria, Glycine max, Nicotiana tabacum, Physcomitrella patens, Prochlorococcus sp. (surface and a deep water ecotype), Spinacia oleracea

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

Wesatern blot with anti-PsaC antibody

2 µg of total protein from Horderum vulgare (1), Chlamydomonas reinhardtii total cell (2)Synechococcus sp. 7942 total cell (3), were extracted with PEB (AS08 300) and separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 50 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in 2% blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with chemiluminescent detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).

Additional information

Additional information

Peptide target used to elicit this antibody is well conserved in all photoautotrophs except some cyanobacteria, some red algae and Cyanophora paradoxa, which contain a conserved substitution of a valine to an isoleucine. The performance of the antibodies has been confirmed against taxa containing both the valine and isoleucine variants.

 

For reconstitution of PsaC antibodies (AS10 939) add 50 µl of sterile water. For reconstitution of PsaC positive control add 100 µl of sterile water.

In some species minor cross reactions with some larger proteins are seen. These may contain related iron-sulfur binding motifs. Therefore size verification of the reacting band is required. Due to the small size of the protein, care should be taken to differentiate between chemiluminescent signal from PsaC and non-specific signals from chlotophylls or lipids if pigment is retained near the bottom of the blot.

Protein standard: use a load of 2 µl per well with ECL detection system and 4 µl per well with alkaline phospatase.

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Background

Background

PsaC is a conserved, chloroplast-encoded, Fe-S binding protein of approximately 10 kDa, present in all known Photosystem I complexes. It is located on the stromal side of the thylacoid membranes. PsaC coordinates the Fe–S clusters FA and FB through two cysteine-rich domains.

Product citations

Selected references Ifuku et al. (2005). PsbP protein, but not PsbQ protein, is essential for the regulation and stabilization of photosystem II in higher plants. Plant Physiol. 3:1175-1184.
Oesterhelt et al (2007). Regulation of photosynthesis in the unicellular acidophilic red alga Galdieria sulphuraria. Plant J.3:500511.

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