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AGO set
Tag Antibody Set 

PsaG | PSI-G subunit of photosystem I

AS04 048  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, N. tabacum, S. oleracea

PsaG | PSI-G subunit of photosystem I in the group Antibodies for Plant/Algal  / Photosynthesis  / PSI (Photosystem I) at Agrisera AB (Antibodies for research) (AS04 048)

PRODUCT INFORMATION IN PDF

Qty: 
332 €

Datasheet Product citations Protocols Add review

Product Information

Immunogen

Fusion protein between DHFR and the mature part of PSI-G of Arabidopsis thaliana, TAIR: At1g55670 in the pQE42 vector

Host Rabbit
Clonality Polyclonal
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunogold (IG), Western blot (WB)
Recommended dilution 1 : 120-1 : 500 (IG), 1 : 2000-1 : 5000 (WB)
Expected | apparent MW

17 | 11 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Nicotiana tabcum, Spinacia oleracea
Predicted reactivity

Pisum sativum

Not reactive in

Hordeum vulgare, Chlamydmonas reinhardtii, Synechococcus sp. PCC7842

Application examples

Application examples

Application example

Western blot detection using anti-PsaG antibodies


2 µg of total protein
from Arabidopsis thaliana leaf extracted (1), Horderum vulgare leaf (2)Chlamydomonas reinhardtii total cell (3), Synechococcus sp. PCC7942 total cell (4), all extracted with PEB (AS08 300) were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% blocking reagent in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:20 000 in 2% blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with chemiluminescence detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).

Additional information

Immunogold localization has been done in a leaf material of Arabidopsis thaliana.

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Background

Background

PsaG is subunit located in the Photosystem I complex. It plats a role in stablizing the binding of the peripheral antenna. PsaG, together with PsaH and PsaN, are unique to higher plants and algae. Immunogen: Fusion protein between DHFR and the mature part of PsaG.

Product citations

Selected references Bock (2012). The plastid genome-encodedYcf4 protein functions as a non-essential assembly factor for photosystem I in higher plants. Plant Physiol. ahead of print.
Jensen et al. (2002) Photosystem I activity is increased in the absence of the PSI-G subunit. J. Biol. Chem. 277: 2798-2803.

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