PsaG | PSI-G subunit of photosystem I
AS04 048 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, N. tabacum, S. oleracea
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Fusion protein between DHFR and the mature part of PSI-G of Arabidopsis thaliana, TAIR: At1g55670 in the pQE42 vector
17 | 11 kDa
Hordeum vulgare, Chlamydmonas reinhardtii, Synechococcus sp. PCC7842
2 µg of total protein from Arabidopsis thaliana leaf extracted (1), Horderum vulgare leaf (2), Chlamydomonas reinhardtii total cell (3), Synechococcus sp. PCC7942 total cell (4), all extracted with PEB (AS08 300) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% blocking reagent in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:20 000 in 2% blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with chemiluminescence detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).
PsaG is subunit located in the Photosystem I complex. It plats a role in stablizing the binding of the peripheral antenna. PsaG, together with PsaH and PsaN, are unique to higher plants and algae. Immunogen: Fusion protein between DHFR and the mature part of PsaG.
Jensen et al. (2002) Photosystem I activity is increased in the absence of the PSI-G subunit. J. Biol. Chem. 277: 2798-2803.