PsbZ | ycf9 protein of PSII
AS06 115 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Pisum sativum
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KLH-conjugated synthetic peptide derived from PsbZ protein sequence of Arabidopsis thaliana (AtCg00300). This sequence is highly conserved in dicots, monocots, and Physcomitrella patens but only weakly in Chlamydomonas reinhardtii.
6.5 | 6.5 kDa for Arabidopsis thaliana
Dicots, Physcomitrella patens
5 µg of total protein from (1) Arabidopsis thaliana leaf extracted with PEB (AS08 300), (2) chloroplast, (3) thylakoids, (4) chloroplasts from high light treated plants, (5) thylakoids from high light treated plants, (6) PSI preparation , (7) Pisum sativum BBY particles were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-PsbZ (AS06 115, 1:1000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent, using a Fuji LAS-3000 CCD (240s, standard sensitivity). There is a strong cross-reaction between 20-30 kDa.
The 6.5 kDa PSII subunit PsbZ (ycf9 in Chlamydomonas) is coded by an open reading frame that is ubiquitously present in chloroplast and cyanobacterial genomes. PsbZ seems to control the interaction of PSII cores with the light-harvesting antenna involving changes in protein phosphorylation within PSII units, the deepoxidation state of xanthophylls, and the kinetics and amplitude of nonphotochemical quenching (NPQ).
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