IKP

Pseudomonas aeruginosa

Product no: IMS01-133-332

IMS01-133-332  |  Clonality: Polyclonal  |  Host: Hen  |  Reactivity: Pseudomonas aeruginosa

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  • Product Info
  • Immunogen:

    whole, formaldehyde killed, bacteria, strains PAO1 and Habs1

    Host: Chicken
    Clonality: Polyclonal
    Purity: Purified, total IgY (chicken egg yolk immunoglobulin) in 0.9% NaCl, 0.02% sodium azide.
    Format: Liquid
    Quantity: 100 l (13.2 mg/ml)
    Storage: Store at 4C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Total, purified IgY
    Recommended dilution: 1 : 1000-1 : 5 000 (ELISA), 1 : 1000-1 : 5 000 (WB)
  • Reactivity
  • Confirmed reactivity: Pseudomonas aeruginosa strains PAO1 and Habs1
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information: The IgY fraction is isolated by a two-step PEG precipitation. procedure followed by ammonium sulphate precipitation.

    The main reactivity of this antibody is flagellin a and b in Pseudomonas, which will lead to variation in positions of detected bands in western blot technique. Nilsson et al. (2007).
    Additional information (application): Reacts with P. aeruginosa strains PAO1 and Habs1; it will also crossreact with other P. aeruginosa strains in ELISA and immunoblotting.

    Antibodies have been tested against E.coli, Klebsiella and Streptococcus aureus and no significant cross-reactivity has been detected.
  • Background
  • Background:

    Pseudomonas aeruginosa is Gram-negative, aerobic, rod-shaped bacteria with unipolar motility. An opportunistic human pathogen, P. aeruginosa is also an opportunistic pathogen of plants. P. aeruginosa bacterias are clinically important because they are resistant to most antibiotics and they are capable of surviving in conditions that few other organisms can tolerate. Pseudomonas is often encountered in hospital and clinical work because it is a major cause of hospital acquired (nosocomal) infections. Its main targets are immunocompromised individuals, burn victims, and individuals on respirators or with precipitation procedure followed by indwelling catheters. Additionally, these pathogens colonize the lungs of cystic fibrosis patients. P. aeruginosa is often identified by its pearlescent appearance and grape-like odor in vitro. Definitive clinical identification of P. aeruginosa includes identifying the production of both pyocyanin and fluorescein as well as its ability to grow at 42°C.

  • Product Citations
  • Selected references: Nilsson et al. (2007). Pseudomonas aeruginosa infections are prevented in cystic fibrosis patients by avian antibodies binding Pseudomonas aeruginosa flagellin. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Sep 1;856(1-2):75-80.doi: 0.1016/j.jchromb.2007.05.029.
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