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Pseudomonas aeruginosa

IMS01-133-332  |  Clonality: Polyclonal  |  Host: Hen  |  Reactivity: Pseudomonas aeruginosa

Pseudomonas aeruginosa in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (IMS01-133-332)

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Product Information

Immunogen

whole, formaldehyde killed, bacteria, strains PAO1 and Habs1

Host Chicken
Clonality Polyclonal
Purity Total IgY fraction
Format Liquid in 0.9% NaCl, 0.1% sodium azide
Quantity 100 µl (13.2 mg/ml)
Storage Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications Total, purified IgY
Recommended dilution 1 : 1000-1 : 5 000 (ELISA), 1 : 1000-1 : 5 000 (WB)

Reactivity

Confirmed reactivity Pseudomonas aeruginosa strains PAO1 and Habs1
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information The IgY fraction is isolated by a two-step PEG precipitation. procedure followed by ammonium sulphate precipitation.

The main reactivity of this antibody is flagellin a and b in Pseudomonas, which will lead to variation in positions of detected bands in western blot technique. Nilsson et al. (2007).
Reacts with P. aeruginosa strains PAO1 and Habs1; it will also crossreact with other P. aeruginosa strains in ELISA and immunoblotting.

Antibodies have been tested against E.coli, Klebsiella and Streptococcus aureus and no significant cross-reactivity has been detected. 

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Secondary antibodies

Background

Background

Pseudomonas aeruginosa is Gram-negative, aerobic, rod-shaped bacteria with unipolar motility. An opportunistic human pathogen, P. aeruginosa is also an opportunistic pathogen of plants. P. aeruginosa bacterias are clinically important because they are resistant to most antibiotics and they are capable of surviving in conditions that few other organisms can tolerate. Pseudomonas is often encountered in hospital and clinical work because it is a major cause of hospital acquired (nosocomal) infections. Its main targets are immunocompromised individuals, burn victims, and individuals on respirators or with precipitation procedure followed by indwelling catheters. Additionally, these pathogens colonize the lungs of cystic fibrosis patients. P. aeruginosa is often identified by its pearlescent appearance and grape-like odor in vitro. Definitive clinical identification of P. aeruginosa includes identifying the production of both pyocyanin and fluorescein as well as its ability to grow at 42°C.

Product citations

Selected references Nilsson et al. (2007). Pseudomonas aeruginosa infections are prevented in cystic fibrosis patients by avian antibodies binding Pseudomonas aeruginosa flagellin. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Sep 1;856(1-2):75-80.doi: 0.1016/j.jchromb.2007.05.029.

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