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Rabbit anti-Human IgM (ĩ chain), ALP conjugated

AS10 1507 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Human IgM (µ chain)

Rabbit anti-Human IgM (ĩ chain), ALP conjugated in the group Secondary Antibodies / Anti-Human / ALP (alkaline phosphatase) / IgM at Agrisera AB (Antibodies for research) (AS10 1507)

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Product Information

Host Rabbit
Clonality Polyclonal
Purity Affinity purified rabbit IgG
Format Liquid
Quantity 0.5 mg
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming
Recommended dilution This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1 : 500-1 : 2000 (IHC), 1 : 50 000-1 : 5 000 (WB)

Reactivity

Application examples

Additional information

Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.50 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.

Based on immunoelectrophoresis, this antibody reacts with: heavy chains on human IgM Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins

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Background

Background Rabbit anti-human IgM (µ chain) is an ALP conjugated secondary antibody which binds to human IgM (µ chain) in immunological assays. Antibody is affinity purified using solid phase human kappa chain.

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