RbcS | Rubisco small subunit (SSU) (affinity purified)

AS07 259A | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, C. sativus, H. vulgare, M. domestica, N. tabacum

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from all known sequences of RbcS from monocots and dicots including RuBisCO small subunit 1B of Arabidopsis thaliana UniProt: P10796, TAIR: At5g38430

Host Rabbit

Clonality Polyclonal

Purity Affinity purified

Format Lyophilized in PBS pH 7.4

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

20 | 15 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Cucumis sativus, Hordeum vulgare, Malus domestica, Nicotiana tabacum

Predicted reactivity

Algae, Camellia oleifera, Erythranthe guttata, Flaveria bidentis, Flaveria sonorensis, Glycine max, L, Marchantia paleacea, Nicotiana benthamiana, Oryza sativa, Petunia hybrida, Polianthes tuberosa, Populus deltoides, Triticum aestivum, Solanum melongena, Solanum tuberosum, Zea mays

Not reactive in Cyanobacteria

Application examples

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Additional information

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matching secondary antibody

Background

Rubisco catalyzes the rate-limiting step of CO₂ fixation in photosynthesis. This enzyme contains two subunits, each present in eight copies. In plants and green algae, 55-kD large subunit is coded by the chloroplast rbcL gene, and the 15-kD small subunit is coded by a family of nuclear RbcS genes.