Rhamnogalacturonan-Ib (clone CCRC-M61)
AS16 3237 | Clonality: monoclonal | Host: Mouse | Reactivity: Arabidopsis thaliana, Sinapsus sp.
From the laboratory of Michael G. Hahn, PhD, University of Georgia
DATA SHEET IN PDF| Data sheet | Product citations | Add review |
Product Information
Immunogen
MeBSA-conjugated Sinapus alba seed mucilage.
Host
Mouse
Clonality
Monoclonal
Subclass/isotype
IgM
Format
Cell culture supernatant, liquid.
Quantity
5 ml
Storage
Antibody can be stored up to 1 month at 4°C, and at -80°C for up to 1 year.
Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications
ELISA (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Recommended dilution
Undiluted or at 1 : 10 (ELISA), (IF), (IHC)
Reactivity
Confirmed reactivity
Arabidopsis thaliana, Camelina sp. , Sinapus sp.
Predicted reactivity
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
Exact working dilution needs to be determined by end user.
CCRC-M61 binds to Camelina seed mucilage and weakly to Glucuronoxylan.
Background
Background
Rhamnogalacturonans (RGs) are pectic polysaccharides found in the cell wall, which contain a repeating dissacharides backbone:
α-D-GalpA-(1,2)-α-L-Rhap-(1)
Product citations
Selected references
Pattathil et al. (2012). Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6.
Patathil et al. (2010). A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiol. 2010 Jun;153(2):514-25.doi: 10.1104/pp.109.151985.
Patathil et al. (2010). A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiol. 2010 Jun;153(2):514-25.doi: 10.1104/pp.109.151985.
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