DISCONTINUED Rnr1 | Ribonucleoside-diphosphate reductase large subunit

AS16 3639 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Saccharomyces cerevisiae

Replaced by AS21 4608

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Saccharomyces cerevisiae Rnr1 protein sequence UniProt: P21524

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes

Tested applications Western blot (WB)

Recommended dilution 1 : 5 000-1 : 10 000 (WB)

Expected | apparent MW 99,56 | 100 kDa

Reactivity

Confirmed reactivity Saccharomyces cerevisiae

Predicted reactivity

Saccharomyces cerevisiae

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

10 ul of total protein from a 1 x 108 cells/ml culture of Saccharomyces cerevisiae extracted with 20% TCA and re-suspended in 1X loading buffer (4X SDS loading buffer: 150 mM TrisHCl pH 7.0, 25% Glycerol, 12% SDS, 0.02% Bromophenol Blue, 5% B-mercaptoethanol) were separated on 7.5% SDS-PAGE and blotted to nitrocellulose membrane for 2h/60V using tank transfer. Blots were blocked (5% milk) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:10000 or 1:5000 o/n at 4°C. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602) diluted to 1:50 000 or 1: 75 000 in 5% milk for 1h at RT with agitation. The blot was washed as above and developed with Western Lightning ECL Pro (PerkinElmer). Exposure time was between 30 seconds and 1 minute depending on the dilutions.

Courtesy of Dr. Isaac Corcoles, NWCR, United Kingdom

Additional information

Related products

AS09 574 | Anti-Rnr3, rabbit antibodies

AS10 847 | Anti-Sml1 | Suppressor of Mec1 lethality, rabbit antibodies

Secondary antibodies

Background

Saccharomyces cerevisiae Rnr1 (EC=1.17.4.1) is an enzyme from ribonucleoside diphosphate reductase large chain family. Is localized to cytoplasm and provides precursors necessary for DNA synthesis. Alternative names: ribonucleotide reductase large subunit 1, ribonucleotide reductase R1 subunit 1

Product citations

Selected references Ros-Carrero et al. (2020). The yeast Aft1 transcription factor activates ribonucleotide reductase catalytic subunit RNR1 in response to iron deficiency. Biochim Biophys Acta Gene Regul Mech. 2020 Mar 6;1863(7):194522. doi: 10.1016/j.bbagrm.2020.194522.
Corcoles-Saez et al. (2019). Functional link between mitochondria and Rnr3, the minor catalytic subunit of yeast ribonucleotide reductase. Microb Cell. 2019 May 20;6(6):286-294. doi: 10.15698/mic2019.06.680.
Sampaio-Marques et al. (2019). α-Synuclein toxicity in yeast and human cells is caused by cell cycle re-entry and autophagy degradation of ribonucleotide reductase 1. Aging Cell. 2019 Aug;18(4):e12922. doi: 10.1111/acel.12922.