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RPL2 | Ribosomal protein L2 (chloroplastic)

AS15 2876  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A.thaliana, H. vulgare, N. Tabacum. S. lycopersicum, S. oleracea 

Back in stock in July 2020

RPL2 | Ribosomal protein L2 (chloroplastic) in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Translation at Agrisera AB (Antibodies for research) (AS15 2876)

DATA SHEET IN PDF

Qty: 
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How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen

Full length, recombinant RPL2 protein from Arabidopsis thaliana, Uniprot: P56791, TAIR:ATCG00830

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage

For reconstitution add 100 µl of sterile water.

Lyophilized antibody can be stored at -20 or -80°C.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Recommended dilution 1 : 3000 (WB)
Expected | apparent MW

29.9 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Spinacia oleracea
Predicted reactivity Brassica napus, Cannabis sativa, Capsella rubella, Citrus sinensis, Cucumis sativus, Gossypium arboreum, Populus trichocarpa, Solanum lycopersicum, Solanum tuberosum, Ricinus communis, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

western blot using anti-plant RPL2 antibodies

20 µg of total protein from Spinacia oleracea (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) leafs were separated on 12 % SDS-PAGE  and blotted 1h to PVDF using tank transfer. Blots were blocked with 5 % milk in TBS  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 5 seconds.

Note: Solanum lycopersicum requires higher load/well and another western blot protocol. If you intend to use this antibody on this species, you are welcome to contact us.

Additional information

The RPL2 protein is a small protein that should be best analysed using 14% SDS PAGE as described by Schägger (2006, Nature Protocols, 1, 16-20).

Following sucrose gradient centrifugation of maize chloroplasts this antibody detects two bands that are very similar in size.

Related products

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collection of antibodies to proteins involved in photosynthesis

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Secondary antibodies

Background

Background Ribosomal protein L2, chloroplastic, is one of the primary proteins involved in rRNA-binding and is a constituent of the large subunit of the ribosomal complex.

Product citations

Selected references Wang et al. (2020) Rerouting of ribosomal proteins into splicing in plant organelles. BioRxiv, DOI: 10.1101/2020.03.03.974766 .
Jeon et al. (2017). Functional characterization of chloroplast-targeted RbgA GTPase in higher plants. Plant Mol Biol. 2017 Nov;95(4-5):463-479. doi: 10.1007/s11103-017-0664-y.

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