RPL4 | ribosomal protein L4 (chloroplastic)
AS15 3076 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, H. vulgare, N. Tabacum. S. lycopersicum, S. oleracea, Z.mays
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Full length, recombinant RPL4 protein from Spinacia oleracea, UniProt: O49937
32 | 26 kDa
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5 µg of total protein from leaf extract (upper panel) or 15 µg of total protein from leaf extract from Spinacia oleracea (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) leafs were separated on 12 % SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with 5 % milk in TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary anti-L4 antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 5 seconds.
Alternative names: EMB2784, EMBRYO DEFECTIVE 2784, PLASTID RIBOSOMAL PROTEIN L4, PRPL4, RIBOSOMAL PROTEIN L4.
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