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RPL4 | ribosomal protein L4 (chloroplastic)

AS15 3076  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A.thaliana, H. vulgare, N. Tabacum. S. lycopersicum, S. oleracea, Z.mays

RPL4 |  ribosomal protein L4 (chloroplastic) in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Translation at Agrisera AB (Antibodies for research) (AS15 3076)

DATA SHEET IN PDF

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How to cite this product:
Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

Full length, recombinant RPL4 protein from Spinacia oleracea, UniProt: O49937

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage

For reconstitution add 100 µl of sterile water.

Lyophilized antibody can be stored at -20 or -80°C.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

32 | 26 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Spinacia oleracea, Solanum lycopersicum, Zea mays
Predicted reactivity Gossypium arboreum, Linum usitatissimum, Medicago truncatula, Morus notabilis, Nicotiana tabacum, Oryza sativa, Theobroma cacao, Ricinus communis, Zostera marina
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

western blot using anti-RPL4 antibodies (chloroplastic)

5 µg of total protein from leaf extract (upper panel) or 15 µg of total protein from leaf extract from Spinacia oleracea (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) leafs were separated on 12 % SDS-PAGE  and blotted 1h to PVDF using tank transfer. Blots were blocked with 5 % milk in TBS  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary anti-L4 antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 5 seconds.

Additional information

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Secondary antibodies

Background

Background Ribosomal protein L4, chloroplastic, is one of the primary proteins involved in rRNA-binding, located in chloroplast.

Alternative names: EMB2784, EMBRYO DEFECTIVE 2784, PLASTID RIBOSOMAL PROTEIN L4, PRPL4, RIBOSOMAL PROTEIN L4.

Product citations

Selected references Swift et al. (2020). Functional Analysis of PSRP1, the Chloroplast Homolog of a Cyanobacterial Ribosome Hibernation Factor. Plants (Basel). 2020 Feb 6;9(2). pii: E209. doi: 10.3390/plants9020209.

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