RpoTm | DNA-directed mitochondrial RNA polymerase (RpoT1; mitochondrial NEP)

AS15 3078  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

RpoTm | DNA-directed mitochondrial RNA polymerase (RpoT1; mitochondrial NEP) in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Transcription regulation at Agrisera AB (Antibodies for research) (AS15 3078)


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Product Information

Immunogen recombinant, N-terminal part of DNA-directed RNA polymerase of Arabidopsis thaliana, UniProt: P92969, TAIR: AT1G68990
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes
Tested applications Western blot (WB)
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW 111,9 | 110 kDa


Confirmed reactivity Arabidopsis thaliana, Nicotiana tabcum, Spinacia oleracea, Solanum lycopersicum
Predicted reactivity Arabis alpina, Amborella trichopoda, Beta vulgaris, Brassica napus, Brassica oleracea, Capsella rubella, Citrus sinensis, CHenopodium album, Coffea canephora, Cucumis sativus, Erythranthe guttata, Eucalyptus grandis, Eutrema salsugineum, Glycine soja, Gossypium raimondii, Medicago truncatula, Morus notabilis, Nicotiana tabacum, Phaseolus vulgaris, Populus trichocarpa, Prunus persica, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

western blot using anti-RPOTm antibodies

2.5 ng (1), 5 ng (2), 10 ng (3) of recombinant Arabidopsis thaliana RPOTm and 40 µg of a total cell extract from Arabidopsis thaliana leaves (4) were separated on 12 % SDS-PAGE  and blotted 1h to nitrocellulose using tank transfer. Blots were blocked with 5 % milk in TBS  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 1 to 5 seconds.

Band at 110 kDa represents fully matured protein.

Additional information

Antibodies works on a total cell extract and purified mitochondria

Related products


Background RPOTm (phage-type mitochondrial RNA polymerase) is a mitochondrial protein, encoded in nucleus which belongs to a small family of nuclear-encoded Phage type RNA polymerases (RPOTs) involved in the transcription of mitochondrial genes in Arabidopsis thaliana.

Product citations

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