RpoTm | DNA-directed mitochondrial RNA polymerase (RpoT1; mitochondrial NEP)
AS15 3078 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
DATA SHEET IN PDF| Data sheet | Product citations | Add review |
Product Information
Immunogen
recombinant, N-terminal part of DNA-directed RNA polymerase of Arabidopsis thaliana, UniProt: P92969, TAIR: AT1G68990
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
100 ĩl
Reconstitution
For reconstitution add 100 ĩl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 2000 (WB)
Expected | apparent MW
111,9 | 110 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana, Nicotiana tabcum, Spinacia oleracea, Solanum lycopersicum
Predicted reactivity
Arabis alpina, Amborella trichopoda, Beta vulgaris, Brassica napus, Brassica oleracea, Capsella rubella, Citrus sinensis, CHenopodium album, Coffea canephora, Cucumis sativus, Erythranthe guttata, Eucalyptus grandis, Eutrema salsugineum, Glycine soja, Gossypium raimondii, Medicago truncatula, Morus notabilis, Nicotiana tabacum, Phaseolus vulgaris, Populus trichocarpa, Prunus persica, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Vitis vinifera
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
application example
2.5 ng (1), 5 ng (2), 10 ng (3) of recombinant Arabidopsis thaliana RPOTm and 40 µg of a total cell extract from Arabidopsis thaliana leaves (4) were separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose using tank transfer. Blots were blocked with 5 % milk in TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 1 to 5 seconds.
Band at 110 kDa represents fully matured protein.
2.5 ng (1), 5 ng (2), 10 ng (3) of recombinant Arabidopsis thaliana RPOTm and 40 µg of a total cell extract from Arabidopsis thaliana leaves (4) were separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose using tank transfer. Blots were blocked with 5 % milk in TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 1 to 5 seconds.
Band at 110 kDa represents fully matured protein.
Additional information
Antibodies works on a total cell extract and purified mitochondria
Background
Background
RPOTm (phage-type mitochondrial RNA polymerase) is a mitochondrial protein, encoded in nucleus which belongs to a small family of nuclear-encoded Phage type RNA polymerases (RPOTs) involved in the transcription of mitochondrial genes in Arabidopsis thaliana.
Product citations
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