RPS1 | Ribosomal protein S1 (chloroplastic)

AS15 2875 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, N. tabacum, S. oleracea, Z. mays

RPS1 | Ribosomal protein S1 (chloroplastic)

Product Information

Immunogen

Recombinant S1 protein from spinach Spinacia oleracea, UniProt: P29344

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water

Storage For reconstitution add 100 µl of sterile water.Lyophilized antibody can be stored at -20 or -80°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW 45,1 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Solanum lycopersicum (with higher load /well), Spinacia oleracea, Zea mays

Predicted reactivity Capsella rubella, Citrus sinensis, Cucumis sativus, Brassica napus, Gossypium arboreum, Populus trichocarpa, Ricinus communis, Solanum tuberosum, Vitis vinifera
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

5 µg of total protein from Spinacia oleracea (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) leafs were separated on 12 % SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with 5 % milk in TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 15 seconds.

RPS1 antibodies will react with Solanum lycopersicum when load per well is increased to 10-15 µg/well.

Additional information

Background

Background Ribosomal protein S1, chloroplastic, is a protein involved in the initiation complex formation via a strong mRNA-binding activity. It has a poly(A)-binding activity, this might play a significant role as a control element in chloroplast mRNA translation.

Product citations

Selected references Wang et al. (2020) Rerouting of ribosomal proteins into splicing in plant organelles. BioRxiv, DOI: 10.1101/2020.03.03.974766 .
Swift et al. (2020). Functional Analysis of PSRP1, the Chloroplast Homolog of a Cyanobacterial Ribosome Hibernation Factor. Plants (Basel). 2020 Feb 6;9(2). pii: E209. doi: 10.3390/plants9020209.
Swift et al. (2020). Functional Analysis of PSRP1, the Chloroplast Homolog of a Cyanobacterial Ribosome Hibernation Factor. Plants (Basel). 2020 Feb 6;9(2). pii: E209. doi: 10.3390/plants9020209.

calculated \| apparent molecular mass [kDa]:	45,1 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	Recombinant S1 protein from spinach Spinacia oleracea, UniProt: P29344
Purity:	Serum
Quantity:	100 µl
recommended dilution:	1 : 1000 (WB)
Reconstitution:	For reconstitution add 100 µl of sterile water
storage:	For reconstitution add 100 µl of sterile water.Lyophilized antibody can be stored at -20 or -80°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
All references:	Wang et al. (2020) Rerouting of ribosomal proteins into splicing in plant organelles. BioRxiv, DOI: 10.1101/2020.03.03.974766 . Swift et al. (2020). Functional Analysis of PSRP1, the Chloroplast Homolog of a Cyanobacterial Ribosome Hibernation Factor. Plants (Basel). 2020 Feb 6;9(2). pii: E209. doi: 10.3390/plants9020209. Swift et al. (2020). Functional Analysis of PSRP1, the Chloroplast Homolog of a Cyanobacterial Ribosome Hibernation Factor. Plants (Basel). 2020 Feb 6;9(2). pii: E209. doi: 10.3390/plants9020209.
background:	Ribosomal protein S1, chloroplastic, is a protein involved in the initiation complex formation via a strong mRNA-binding activity. It has a poly(A)-binding activity, this might play a significant role as a control element in chloroplast mRNA translation.
Confirmed reactivity:	Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Solanum lycopersicum (with higher load /well), Spinacia oleracea, Zea mays
predicted reactivity:	Capsella rubella, Citrus sinensis, Cucumis sativus, Brassica napus, Gossypium arboreum, Populus trichocarpa, Ricinus communis, Solanum tuberosum, Vitis vinifera Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	application example 5 µg of total protein from Spinacia oleracea (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) leafs were separated on 12 % SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with 5 % milk in TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 15 seconds. RPS1 antibodies will react with Solanum lycopersicum when load per well is increased to 10-15 µg/well.

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