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RPS5 | Ribosomal protein S5 (chloroplastic)

AS15 3075  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, H. vulgare, N. tabacum, S. lycopersicum, S. oleracea

RPS5 | Ribosomal protein S5 (chloroplastic) in the group Antibodies Plant/Algal  / DNA/RNA/Cell Cycle / Translation at Agrisera AB (Antibodies for research) (AS15 3075)
RPS5 | Ribosomal protein S5 (chloroplastic)



DATA SHEET IN PDF

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Product Information

Immunogen

Recombinant S5 protein from Spinacia oleracea, UniProt: Q9ST69

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water
Storage For reconstitution add 100 ĩl of sterile water.Lyophilized antibody can be stored at -20 or -80°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW 27,8 | 27,5 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Solanum lycopersicum (with higher load /well), Spinacia oleracea
Predicted reactivity Algae, Arthrospira platensis, Beta vulgaris, Glycine soja, Gossypium arboreum, Medicago trunCatul, Morus notabilis, Oryza sativa, Phaseolus vulgaris, Riciunus communis, Theobroma cacao, Zea mays, Zostera marin
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

western blot using anti-RPS5 antibodies

5 µg of total protein from Spinacia oleracea (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) leafs were separated on 12 % SDS-PAGE  and blotted 1h to PVDF using tank transfer. Blots were blocked with 5 % milk in TBS  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary anti-S5 antibody at a dilution of 1: 2 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 15 seconds.

RPS5 antibodies will react with Solanum lycopersicum when load per well is increased to 10-15 µg/well.

Additional information

Related products

Background

Background Ribosomal protein S5, chloroplastic is a ribosomal protein from S5 family protein. It functions as a structural constituent of ribosome, RNA binding. It is involved in: response to cadmium ion, response to cold and translation.

Product citations

Selected references Xiong et al. (2022) a chloroplast nucleoid protein of bacterial origin linking chloroplast transcriptional and translational machineries, is required for proper chloroplast gene expression in Arabidopsis thaliana. Nucleic Acids Res. 2022 Jun 23;50(12):6715-34. doi: 10.1093/nar/gkac501. Epub ahead of print. PMID: 35736138; PMCID: PMC9262611.
Schmid et al. (2018). PUMPKIN, the sole Plastid UMP Kinase, Associates with Group II Introns and Alters Their Metabolism. Plant Physiol. 2018 Nov 8. pii: pp.00687.2018. doi: 10.1104/pp.18.00687.
calculated | apparent molecular mass [kDa]: 27,8 | 27,5 kDa
Clonality: Polyclonal
Format: Lyophilized
Host: Rabbit
immunogen:

Recombinant S5 protein from Spinacia oleracea, UniProt: Q9ST69

Purity: Serum
Quantity: 100 ĩl
recommended dilution: 1 : 2000 (WB)
Reconstitution: For reconstitution add 100 ĩl of sterile water
storage: For reconstitution add 100 ĩl of sterile water.Lyophilized antibody can be stored at -20 or -80°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Western blot (WB)
Picture (footer): Application example

western blot using anti-RPS5 antibodies

5 µg of total protein from Spinacia oleracea (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) leafs were separated on 12 % SDS-PAGE  and blotted 1h to PVDF using tank transfer. Blots were blocked with 5 % milk in TBS  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary anti-S5 antibody at a dilution of 1: 2 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 15 seconds.

RPS5 antibodies will react with Solanum lycopersicum when load per well is increased to 10-15 µg/well.
background: Ribosomal protein S5, chloroplastic is a ribosomal protein from S5 family protein. It functions as a structural constituent of ribosome, RNA binding. It is involved in: response to cadmium ion, response to cold and translation.
Confirmed reactivity: Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Solanum lycopersicum (with higher load /well), Spinacia oleracea
predicted reactivity: Algae, Arthrospira platensis, Beta vulgaris, Glycine soja, Gossypium arboreum, Medicago trunCatul, Morus notabilis, Oryza sativa, Phaseolus vulgaris, Riciunus communis, Theobroma cacao, Zea mays, Zostera marin
Species of your interest not listed? Contact us
not reactive in: No confirmed exceptions from predicted reactivity are currently known
All references: Xiong et al. (2022) a chloroplast nucleoid protein of bacterial origin linking chloroplast transcriptional and translational machineries, is required for proper chloroplast gene expression in Arabidopsis thaliana. Nucleic Acids Res. 2022 Jun 23;50(12):6715-34. doi: 10.1093/nar/gkac501. Epub ahead of print. PMID: 35736138; PMCID: PMC9262611.
Schmid et al. (2018). PUMPKIN, the sole Plastid UMP Kinase, Associates with Group II Introns and Alters Their Metabolism. Plant Physiol. 2018 Nov 8. pii: pp.00687.2018. doi: 10.1104/pp.18.00687.

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