RPS5 | Ribosomal protein S5 (chloroplastic)
AS15 3075 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, N. tabacum, S. lycopersicum, S. oleracea
|Recommended dilution||1 : 2000 (WB)|
|Expected | apparent MW||
27.8 | 27.5 kDa
|Confirmed reactivity||Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Solanum lycopersicum (with higher load /well), Spinacia oleracea|
|Predicted reactivity||Algae, Arthrospira platensis, Beta vulgaris, Glycine soja, Gossypium arboreum, Medicago trunCatul, Morus notabilis, Oryza sativa, Phaseolus vulgaris, Riciunus communis, Theobroma cacao, Zea mays, Zostera marin|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
|Selected references||to be added when available, antibody released in December 2015|
5 µg of total protein from Spinacia oleracea (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) leafs were separated on 12 % SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with 5 % milk in TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary anti-S5 antibody at a dilution of 1: 2 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 15 seconds.
RPS5 antibodies will react with Solanum lycopersicum when load per well is increased to 10-15 µg/well.
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