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RPS7 | Ribosomal protein S7 (chloroplastic)

AS15 2877  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, H. vulgare, N. tabacum, S. lycopersicum, S. oleracea

RPS7 | Ribosomal protein S7 (chloroplastic) in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Translation at Agrisera AB (Antibodies for research) (AS15 2877)

DATA SHEET IN PDF

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399
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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

full length, recombinant S7 protein from Arabidopsis thaliana, UniProt: P61841, TAIR: ATCG00900

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage

For reconstitution add 100 µl of sterile water.

Lyophilized antibody can be stored at -20 or -80°C.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Recommended dilution 1 : 3000 (WB)
Expected | apparent MW

17.4 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Nicotiana tabacum,Hordeum vulgare, Spinacia oleracea
Predicted reactivity Brassica napus, Cannabis sativa, Capsella rubella, Citrus sinensis, Cucumis sativus, Gossypium arboreum, Ricinus communis, Populus trichocarpa, Solanum lycopersicum, Solanum tuberosum, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

western blot using anti-plant RPS7 antibodies

20 µg of total protein from Spinacia oleracea (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) leafs were separated on 12 % SDS-PAGE  and blotted 1h to PVDF using tank transfer. Blots were blocked with 5 % milk in TBS  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 10 seconds.

Additional information

Related products

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Secondary antibodies

Background

Background Ribosomal protein S7, chloroplastic, is a protein that is a part of the small subunit of the ribosomal complex. It is one of the primary rRNA binding proteins.

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