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Saporin

AS13 2688 | Clonality: Polyclonal | Host: Rabbit | Reactivity: purified saporin

Saporin in the group Antibodies for Human/Animal  / Human Proteins / Cancer at Agrisera AB (Antibodies for research) (AS13 2688)

DATA SHEET IN PDF

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379
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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

Saporin, whole molecule

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage For long term stability requires storage, preferably in small aliquots at -20ºC or lower.It is recommended that a thawed sample is stored at 4ºC for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.
Tested applications ELISA (ELISA), Immunihistochemistry (IHC), Immunofluorescence (IF), Western blot (WB)
Recommended dilution 1 : 200-1 : 2000 is recommended. Optimal dilutions/concentrations should be determined by the end user.
Expected | apparent MW

29.5 kDa

Reactivity

Confirmed reactivity Purified saporin

Application examples

Application examples Application example





Western blot analysis using rabbit polyclonal anti-saporin antisera (AS13 2688). HRP-labelled sheep anti-rabbit was used as the secondary antibody and the immunocomplex was then visualised by DAB. The expected molecular weight of the purified saporin is 29.5 kDa. SC: Spinal Cord homogenate (rat).

Additional information

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Background

Background

Saporin is a ribosome-inactivating protein (RIP) of type I. This monomeric RNA N-glycosidase purified from seeds of the plant Saponaria officinalis also known as Soapwort, is capable of specific depurination of eukaryotic ribosomes thus arresting protein synthesis. No ligand has been identified in saporin hence its inability to transverse the cell membrane. Due to its toxicity and stability of the structure, saporin has proven extremely useful for construction of immunotoxins.

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