AS13 2688 | Clonality: Polyclonal | Host: Rabbit | Reactivity: purified saporin
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Saporin, whole molecule
Western blot analysis using rabbit polyclonal anti-saporin antisera (AS13 2688). HRP-labelled sheep anti-rabbit was used as the secondary antibody and the immunocomplex was then visualised by DAB. The expected molecular weight of the purified saporin is 29.5 kDa. SC: Spinal Cord homogenate (rat).
Saporin is a ribosome-inactivating protein (RIP) of type I. This monomeric RNA N-glycosidase purified from seeds of the plant Saponaria officinalis also known as Soapwort, is capable of specific depurination of eukaryotic ribosomes thus arresting protein synthesis. No ligand has been identified in saporin hence its inability to transverse the cell membrane. Due to its toxicity and stability of the structure, saporin has proven extremely useful for construction of immunotoxins.
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