Saporin
AS13 2688 | Clonality: Polyclonal | Host: Rabbit | Reactivity: purified saporin
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Product Information
Immunogen
Saporin, whole molecule
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
100 µl
Reconstitution
For reconstitution add 100 µl of sterile water.
Storage
For long term stability requires storage, preferably in small aliquots at -20ºC or lower.It is recommended that a thawed sample is stored at 4ºC for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.
Tested applications
ELISA (ELISA), Immunihistochemistry (IHC), Immunofluorescence (IF), Western blot (WB)
Recommended dilution
1 : 200-1 : 2000 is recommended. Optimal dilutions/concentrations should be determined by the end user.
Expected | apparent MW
29.5 kDa
Reactivity
Confirmed reactivity
Purified saporin
Application examples
Application examples
Application example
Western blot analysis using rabbit polyclonal anti-saporin antisera (AS13 2688). HRP-labelled sheep anti-rabbit was used as the secondary antibody and the immunocomplex was then visualised by DAB. The expected molecular weight of the purified saporin is 29.5 kDa. SC: Spinal Cord homogenate (rat).
Western blot analysis using rabbit polyclonal anti-saporin antisera (AS13 2688). HRP-labelled sheep anti-rabbit was used as the secondary antibody and the immunocomplex was then visualised by DAB. The expected molecular weight of the purified saporin is 29.5 kDa. SC: Spinal Cord homogenate (rat).
Additional information
Background
Background
Saporin is a ribosome-inactivating protein (RIP) of type I. This monomeric RNA N-glycosidase purified from seeds of the plant Saponaria officinalis also known as Soapwort, is capable of specific depurination of eukaryotic ribosomes thus arresting protein synthesis. No ligand has been identified in saporin hence its inability to transverse the cell membrane. Due to its toxicity and stability of the structure, saporin has proven extremely useful for construction of immunotoxins.
Product citations
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