SCGB2A2 | Mammaglobin-A

Name: SCGB2A2 | Mammaglobin-A
SKU: AS03 029
Price: 424 EUR
Availability: InStock

AS03 029 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human

SCGB2A2 | Mammaglobin-A in the group Antibodies Human Cell Biology / Cancer at Agrisera AB (Antibodies for research) (AS03 029)

Product Information

Immunogen

KLH-conjugated 16 amino acids-long synthetic peptide derived from C-terminal sequence of human mammaglobin Q13296

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 100 µg

Reconstitution For reconstitution add 238 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunohistochemistry (IHC), Formalin/PFA-fixed paraffin-embedded sections (IHC-P), Western blot (WB)

Recommended dilution 1 µg/ml (IHC-P), 1 µg/ml (WB)

Expected | apparent MW 10,5 | 15 kDa

Reactivity

Confirmed reactivity Human

Predicted reactivity Human

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information No cross-reactivity to lipophilin A, lipophilin B (BU101), lipophilin C (lacryglobin, mammaglobin B), or uteroglobin (Clara cell protein, Clara cell 10 kDa protein) by Western blot analysis of bacterially produced recombinant proteins, Western blot analysis of human breast cancer cells MDA-MB-415 reveals only the mammaglobin band of approximately 15 kDa, Analysis of other cells and tissues might, however, upon prolonged exposure, reveal additional bands at higher molecular weight

Background

Mammaglobin is a 93 amino acids peptide belonging to the secretoglobin superfamily. Mammaglobin is expressed by normal breast and skin tissues and is over-expressed in many human breast cancer tumours. In breast tissue, mammaglobin is expressed as a secreted heterodimer together with the other secretoglobin member lipophilin B. Protein synonymes: Mammaglobin-1, Secretoglobin family 2A member 2

Product citations

Selected references Fischer et al. (2014). Differential expression of secretoglobins in normal ovary and in ovarian carcinoma – Overexpression of mammaglobin-1 is linked to tumor progression. Arch Biochem Biophys. 2014 Mar 3. pii: S0003-9861(14)00077-0. doi: 10.1016/j.abb.2014.02.012.
Sjödin et al (2008). Mammaglobin and lipophilin B expression in breast tumors and their lack of effect on breast cancer cell proliferation. Anticancer Res. 28:1493-1498.

background:	Mammaglobin is a 93 amino acids peptide belonging to the secretoglobin superfamily. Mammaglobin is expressed by normal breast and skin tissues and is over-expressed in many human breast cancer tumours. In breast tissue, mammaglobin is expressed as a secreted heterodimer together with the other secretoglobin member lipophilin B. Protein synonymes: Mammaglobin-1, Secretoglobin family 2A member 2
calculated \| apparent molecular mass [kDa]:	10,5 \| 15 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	KLH-conjugated 16 amino acids-long synthetic peptide derived from C-terminal sequence of human mammaglobin Q13296
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Quantity:	100 µg
recommended dilution:	1 µg/ml (IHC-P), 1 µg/ml (WB)
Reconstitution:	For reconstitution add 238 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Immunohistochemistry (IHC), Formalin/PFA-fixed paraffin-embedded sections (IHC-P), Western blot (WB)
All references:	Fischer et al. (2014). Differential expression of secretoglobins in normal ovary and in ovarian carcinoma – Overexpression of mammaglobin-1 is linked to tumor progression. Arch Biochem Biophys. 2014 Mar 3. pii: S0003-9861(14)00077-0. doi: 10.1016/j.abb.2014.02.012. Sjödin et al (2008). Mammaglobin and lipophilin B expression in breast tumors and their lack of effect on breast cancer cell proliferation. Anticancer Res. 28:1493-1498.
Confirmed reactivity:	Human
predicted reactivity:	Human
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	No cross-reactivity to lipophilin A, lipophilin B (BU101), lipophilin C (lacryglobin, mammaglobin B), or uteroglobin (Clara cell protein, Clara cell 10 kDa protein) by Western blot analysis of bacterially produced recombinant proteins, Western blot analysis of human breast cancer cells MDA-MB-415 reveals only the mammaglobin band of approximately 15 kDa, Analysis of other cells and tissues might, however, upon prolonged exposure, reveal additional bands at higher molecular weight