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SCGB2A2 | Mammaglobin-A

AS03 029  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Human

SCGB2A2 | Mammaglobin-A in the group Antibodies Human Cell Biology / Cancer at Agrisera AB (Antibodies for research) (AS03 029)



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Product Information

Immunogen

KLH-conjugated 16 amino acids-long synthetic peptide derived from C-terminal sequence of human mammaglobin Q13296

Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 100 ĩg
Reconstitution For reconstitution add 238 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Immunohistochemistry (IHC), Formalin/PFA-fixed paraffin-embedded sections (IHC-P), Western blot (WB)
Recommended dilution 1 µg/ml (IHC-P), 1 µg/ml (WB)
Expected | apparent MW 10,5 | 15 kDa

Reactivity

Confirmed reactivity Human
Predicted reactivity Human
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information No cross-reactivity to lipophilin A, lipophilin B (BU101), lipophilin C (lacryglobin, mammaglobin B), or uteroglobin (Clara cell protein, Clara cell 10 kDa protein) by Western blot analysis of bacterially produced recombinant proteins, Western blot analysis of human breast cancer cells MDA-MB-415 reveals only the mammaglobin band of approximately 15 kDa, Analysis of other cells and tissues might, however, upon prolonged exposure, reveal additional bands at higher molecular weight

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AS03 029S | Mammaglobin blocking peptide

Background

Background

Mammaglobin is a 93 amino acids peptide belonging to the secretoglobin superfamily. Mammaglobin is expressed by normal breast and skin tissues and is over-expressed in many human breast cancer tumours. In breast tissue, mammaglobin is expressed as a secreted heterodimer together with the other secretoglobin member lipophilin B. Protein synonymes: Mammaglobin-1, Secretoglobin family 2A member 2

Product citations

Selected references Fischer et al. (2014). Differential expression of secretoglobins in normal ovary and in ovarian carcinoma – Overexpression of mammaglobin-1 is linked to tumor progression. Arch Biochem Biophys. 2014 Mar 3. pii: S0003-9861(14)00077-0. doi: 10.1016/j.abb.2014.02.012.
Sjödin et al (2008). Mammaglobin and lipophilin B expression in breast tumors and their lack of effect on breast cancer cell proliferation. Anticancer Res. 28:1493-1498.

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