SOD1 aa 03-20 | superoxide dismutase 1, soluble

Product no: AS09 534

AS09 534 | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Human

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from human SOD1 protein sequence P00441

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 100 l
    Reconstitution: For reconstitution add 100 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 10 000 (WB)
    Expected | apparent MW: 15,9 kDa
  • Reactivity
  • Confirmed reactivity: Human
    Predicted reactivity: Bovine, Goat, Crab, Guinea Pig, Horse, Mouse, Pig, Rat, Sheep, Yeast
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example 

    western blot using anti-SOD1 antibodieswestern blot using anti-SOD1 antibodies

    10 µl of control mouse brain and a human spinal ventral horn were extracted in 25 volumes of PBS, diluted 1+1 in a sample buffer have been loaded per well and separated on 15% SDS-PAGE and blotted for 1h to PVDF. Blots were blocked immediately following transfer in 5% Dry milk for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 75 000 (AS09 534) for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 5 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:42 000 for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager. Exposure time was 30-600 seconds.

  • Additional Information
  • Additional information (application): Peptide used to elicit this antibody is not conserved in SOD2,3 and 4,
  • Background
  • Background:

    SOD1 [Cu-Zn] (EC=1.15.1.1) is a cytoplasm localized oxidoreductase which destroys radicals normally produced within the cells and toxic to biological systems. Alternative names: SOD, soluble, indophenoloxidase A, Cu/Zn superoxide dismutase, superoxide dismutase, cytosolic

  • Protocols
  • Antibody protocols
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AS16 ECL-N |  low pico to mid femtogram detection

This product can be purchased in 3 different volumes:

AS16 ECL-N-10, 10 ml. Trial size limited to one per customer

AS16 ECL-N-100, 100 ml

AS16 ECL-N-1L, 1L

Choose the appropriate volume in the drop down menu to the right

From 12 €
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