showForm Anti-SOD1 aa 03-20 | superoxide dismutase 1, soluble antibodies

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SOD1 aa 03-20 | superoxide dismutase 1, soluble

AS09 534 | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Human

SOD1 aa 03-20 | superoxide dismutase 1, soluble  in the group Antibodies for Human/Animal  / Human Proteins / Oxidative stress at Agrisera AB (Antibodies for research) (AS09 534)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from human SOD1 protein sequence P00441

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 50 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 10 000 (WB)
Expected | apparent MW

15.9 kDa

Reactivity

Confirmed reactivity Human
Predicted reactivity Bovine, Goat, Crab, Guinea Pig, Horse, Mouse, Pig, Rat, Sheep, Yeast
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example 

western blot using anti-SOD1 antibodieswestern blot using anti-SOD1 antibodies

10 µl of control mouse brain and a human spinal ventral horn were extracted in 25 volumes of PBS, diluted 1+1 in a sample buffer have been loaded per well and separated on 15% SDS-PAGE and blotted for 1h to PVDF. Blots were blocked immediately following transfer in 5% Dry milk for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 75 000 (AS09 534) for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 5 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:42 000 for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager. Exposure time was 30-600 seconds.

Additional information

Peptide used to elicit this antibody is not conserved in SOD2,3 and 4. 

Related products

Related products

AS09 535 | Anti-SOD1 aa 24-39 | superoxide dismutase 1, soluble, rabbit antibodies

AS09 536 | Anti-SOD1 aa 43-57 | superoxide dismutase 1, soluble, rabbit antibodies

AS09 537 | Anti-SOD1 aa 58-72 | superoxide dismutase 1, soluble, rabbit antibodies

AS09 538 | Anti-SOD1 aa 80-96 | superoxide dismutase 1, soluble, rabbit antibodies

AS09 539 | Anti-SOD1 aa 100-115 | superoxide dismutase 1, soluble, rabbit antibodies

AS09 540 | Anti-SOD1 aa 131-153 | superoxide dismutase 1, soluble, rabbit antibodies

Secondary antibodies

Background

Background

SOD1 [Cu-Zn] (EC=1.15.1.1) is a cytoplasm localized oxidoreductase which destroys radicals normally produced within the cells and toxic to biological systems. Alternative names: SOD, soluble, indophenoloxidase A, Cu/Zn superoxide dismutase, superoxide dismutase, cytosolic

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