SOD1 aa 131-153 | superoxide dismutase 1, soluble

Name: SOD1 aa 131-153 | superoxide dismutase 1, soluble
SKU: AS09 540
Price: 394 EUR
Availability: InStock

AS09 540 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human

SOD1 aa 131-153 | superoxide dismutase 1, soluble in the group Antibodies Human Cell Biology / Oxidative stress at Agrisera AB (Antibodies for research) (AS09 540)

Product Information

Immunogen

KLH-conjugated synthetic peptide, amino acids 131-153 derived from human SOD1 protein sequence P00441

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 75 000 (WB)

Expected | apparent MW 15,9 kDa

Reactivity

Confirmed reactivity Human

Predicted reactivity Atlantic Salmon, Bovine, Chimpanzee, Goat, Guinea Pig, Mouse, Pig, Rabbit, Rat, Schizosaccharomyces pombe, Sheep

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application example

western blot using anti-SOD1 antibodies aa 135-153

10 µl of control mouse brain and a human spinal ventral horn were extracted in 25 volumes of PBS, diluted 1+1 in a sample buffer have been loaded per well and separated on 15% SDS-PAGE and blotted for 1h to PVDF. Blots were blocked immediately following transfer in 5% Dry milk for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 75 000 (AS09 540) for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 5 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:42 000 for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager. Exposure time was 30-600 seconds.

Additional information

Peptide used to elicit this antibody is not conserved in SOD2,3 and 4

Background

SOD1 [Cu-Zn] (EC=1.15.1.1) is a cytoplasm localized oxidoreductase which destroys radicals normally produced within the cells and toxic to biological systems. Alternative names: SOD, soluble, indophenoloxidase A, Cu/Zn superoxide dismutase, superoxide dismutase, cytosolic

Product citations

Selected references Kiskinis et al. (2014). Pathways Disrupted in Human ALS Motor Neurons Identified through Genetic Correction of Mutant SOD1. Cell Stem Cell. 2014 Apr 2. pii: S1934-5909(14)00098-8. doi: 10.1016/j.stem.2014.03.004.

background:	SOD1 [Cu-Zn] (EC=1.15.1.1) is a cytoplasm localized oxidoreductase which destroys radicals normally produced within the cells and toxic to biological systems. Alternative names: SOD, soluble, indophenoloxidase A, Cu/Zn superoxide dismutase, superoxide dismutase, cytosolic
calculated \| apparent molecular mass [kDa]:	15,9 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	KLH-conjugated synthetic peptide, amino acids 131-153 derived from human SOD1 protein sequence P00441
Purity:	Serum
Quantity:	50 µl
recommended dilution:	1 : 75 000 (WB)
Reconstitution:	For reconstitution add 50 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
Picture (footer):	Application example 10 µl of control mouse brain and a human spinal ventral horn were extracted in 25 volumes of PBS, diluted 1+1 in a sample buffer have been loaded per well and separated on 15% SDS-PAGE and blotted for 1h to PVDF. Blots were blocked immediately following transfer in 5% Dry milk for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 75 000 (AS09 540) for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 5 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:42 000 for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager. Exposure time was 30-600 seconds.
additional information (application):	Peptide used to elicit this antibody is not conserved in SOD2,3 and 4
Confirmed reactivity:	Human
predicted reactivity:	Atlantic Salmon, Bovine, Chimpanzee, Goat, Guinea Pig, Mouse, Pig, Rabbit, Rat, Schizosaccharomyces pombe, Sheep
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
All references:	Kiskinis et al. (2014). Pathways Disrupted in Human ALS Motor Neurons Identified through Genetic Correction of Mutant SOD1. Cell Stem Cell. 2014 Apr 2. pii: S1934-5909(14)00098-8. doi: 10.1016/j.stem.2014.03.004.