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Product Information
Immunogen
Synthetic spermine conjugated to BSA
Host
Rabbit
Clonality
Polyclonal
Purity
Total IgG
Format
Lyophilized in PBS, Phosphate 10 mmol/l ; NaCl 150 mmol/l at pH 7.35
Quantity
100 µl
Reconstitution
For reconstitution add 50 µl of sterile water and 50 µl of glycerol
Storage
Store lyophilized antibodies at 4°C; after reconstitution stode at -20°C. This aliquote is stable for at least 2 years. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
InDirect ELISA (ELISA) with conjugated spermine
Recommended dilution
The optimal working dilution should be determined by the investigator.
Expected | apparent MW
202.34 g/mol
Reactivity
Confirmed reactivity
Conjugated spermine
Predicted reactivity
Spermine
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
Specificity has been determined using ELISA competition experiments using conjugate of spermine
| Compounds | Cross-reactivity ratio (a) | |
| Spermine-GA-BSA | 1 | |
| Spermidine-GA-BSA | 1/ 3 | |
| Ornithine-GA-BSA | 1/ 500 | |
| Diamino-Pentane-G-BSA | 1/ 700 |
(a) : Spermine-GA-BSA concentration/other conjugated polyamine concentration at half displacement
G = Glutaraldehyde, GA = Glutaric Anhydride, BSA = Bovine Serum Albumin.
Antibodies have very weak reactivity to spermidine and no reactivity to putrescine.
For example of immunocytochemistry protocol check here total IgG was purified using ammonium sulfate precipitation.
Background
Background
Spermine is a positively charged polyamine found in a wide variety of tissues. Alternative names: gerontine, musculamine, neuridine
Product citations
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Agrisera AB Box 57, SE-911 21 Vännäs, SWEDEN | Phone: +46(0)935 33000 | Fax: +46(0)935 33044 | 
