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Spermine

AS09 599  |  Clonality: Polyclonal  |  Host: Rabbit

Limited stock.

Spermine in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Transcription regulation at Agrisera AB (Antibodies for research) (AS09 599)

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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

Synthetic spermine conjugated to BSA

Host Rabbit
Clonality Polyclonal
Purity Total IgG
Format Lyophilized in PBS, Phosphate 10 mmol/l ; NaCl 150 mmol/l at pH 7.35
Quantity 100 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water and 50 ĩl of glycerol
Storage Store lyophilized antibodies at 4°C; after reconstitution stode at -20°C. This aliquote is stable for at least 2 years. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications InDirect ELISA (ELISA) with conjugated spermine
Recommended dilution The optimal working dilution should be determined by the investigator.
Expected | apparent MW

202.34 g/mol

Reactivity

Confirmed reactivity Conjugated spermine
Predicted reactivity Spermine
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

Specificity has been determined using ELISA competition experiments using conjugate of spermine


Compounds Cross-reactivity ratio (a)
   
Spermine-GA-BSA 1
Spermidine-GA-BSA 1/ 3
Ornithine-GA-BSA 1/ 500
Diamino-Pentane-G-BSA 1/ 700

(a) : Spermine-GA-BSA concentration/other conjugated polyamine concentration at half displacement
G = Glutaraldehyde, GA = Glutaric Anhydride, BSA = Bovine Serum Albumin.

Antibodies have very weak reactivity to spermidine and no reactivity to putrescine.

For example of immunocytochemistry protocol check here total IgG was purified using ammonium sulfate precipitation.

Related products

Related products

AS09 598 | anti-ornithine rabbit antibodies

Secondary antibodies

Background

Background

Spermine is a positively charged polyamine found in a wide variety of tissues. Alternative names: gerontine, musculamine, neuridine

Product citations

Selected references

to be added when available